Liquid chromatography niacin

More recently [635], a unique extraction step in supplemented foods, by using hot water and a precipitation solution, following by HPLC-ELD/UV analysis has been performed for the simultaneous determination of pyridoxine, thiamine, riboflavin, niacin, pantothenic acid, folic acid, cyanoco-balamin, and ascorbic acid. The mobile phase consisting of phosphate buffer and methanol has been modified in order to perform ion-liquid chromatography by adding l-octanesulfonic acid sodium salt. Furthermore, triethylamine has been also added to improve peak symmetry. 

Sandhu JS, Fraser DR. Measurement of niacin metabolites in urine by high pressure liquid chromatography. A simple, sensitive assay of niacin nutritional status. Int J Vitam Nutr Res 1981 51 139-44. 

Ward, C. M., and Trenerry, V. C., The determination of niacin in cereals, meat and selected foods by capillary electrophoresis and high performance liquid chromatography. Food Chem., 60, 667, 1997. 

Diaz-Pollan, C., and Vidal-Valverde, C., Niacin determination in legumes by capillary electrophoresis (CE). Comparison with high performance liquid chromatography (HPLC), J. High Resolut. 

Table 19.3 Results of niacin determinations for milk samples. Niacin determinations by liquid chromatography-isotope dilution mass spectrometry (LC-IDMS) are compared to expected values for four milk samples. Expected niacin levels for milk are roughly 1 ppm, according to the USDA Nutrient Database for Standard Reference (US Department of Agriculture 2010) and results obtained for two commercial milk samples (Brands F and G) are a little under 1 ppm. The result for sample NFY0409F6 is about 30% lower, but is consistent with results obtained for other milk samples from the same source. In addition, the niaein level for NFY0409F6 was estimated by a standard additions experiment, the result from which is in agreement with the estimate from the normal LC-IDMS procedure. The level obtained for the reference material (RM) RM 8435 whole milk powder, reported on a dry mass basis, is in agreement with the reference value. Data are from Goldschmidt and Wolf (2007), with permission from the publisher.

This chapter is about the determination of niacin in foods using isotope dilution mass spectrometry (IDMS) and liquid chromatography (LC). Niacin, also called vitamin B3, refers to two vitamer forms nicotinic acid and niacinamide. 

Goldschmidt, R.J., and Wolf, W.R., 2007. Determination of niacin in food materials by liquid chromatography using isotope dilution mass spectrometry. Journal of AO AC International. 90 1084-1089. 

Juraja, S.M., Trenerry, V.C., Millar, R.G., Scheelings, P., and Buick, D.R., 2003. Asia Pacific Food Analysis Network (APNFAN) Training Exercise The determination of niacin in cereals by alkaline extraction and high performance liquid chromatography. Journal of Food Composition and Analysis. 16 93-106. 

LaCroix, D.E., Wolf, W.R., and Vanderslice, J.T., 1999. Determination of niacin in infant formula and wheat flour by anion-exchange liquid chromatography with solid phase extraction cleanup. Journal of AOAC International. 82 128-133. 

Rizzolo, A., Baldo, C., and Polesello, A., 1991. Application of high-performance liquid chromatography to the analysis of niacin and biotin in italian almond cultivars. Journal of Chromatography. 553 187-192. 

Tyler, T.A., and Shrago, R.R., 1980. Determination of niacin in cereal samples by HPLC. Journal of Liquid Chromatography. 3 269-277. 

Kirchmeier, R.L. and Upton, R.P., 1978. Simultaneous determination of niacin, niacinamide, pyridoxine, thiamine, and riboflavin in multivitamin blends by ion-pair high-pressure liquid chromatography. Journal of Pharmaceutical Sciences. 67 1444 1446. 

S Hirayama, M Maruyama. Determination of a small amount of niacin in foodstuffs by high-performance liquid chromatography. J Chromatogr 588 171-175, 1991. 

EG Carter. Quantitation of urinary niacin metabolites by reversed-phase liquid chromatography. Am J Clin Nulr 36 926-930, 1982. 

WG Chase, AM Soliman. Analysis of thiamin, riboflavin, pyridoxine and niacin in multivitamin premixes and supplements by high performance liquid chromatography. J Micronutr Anal 7 15-25, 1990. 

LaCroix DE, Wolf WR, Porter E, Cantellops D, Chase Jr GW, Woollard D. Determination of niacin in infant formula by solid-phase extraction and anion-exchange liquid chromatography. J AOAC Int 2001 84(3) 789-804. 

Vidal-Valverde C, Reche A. Determination of available niacin in legumes and meat by high performance liquid chromatography. J Agri Food Chem 1991 39(1) 116—21. 

Lahely S, Bergaentzle M, Hasselmann C. Fluorimetric determination of niacin in foods by high-performance liquid chromatography with post-column derivatization. Food Chem 1999 65(l) 129-33. 

Hamano, T., Mitsuhashi, Y., Aoki, N., and Yamamoto, S., Simultaneous determination of niacin and niacinamide in meats by high-performance liquid chromatography, J. Chromatogr., 457, 403-408, 1988. 

At present, niacin status is most commonly assessed by the assay of some of the breakdown products of niacin coenzymes in the urine. Of these, -methyl nicotinamide (NMN) is the easiest to measure, because of a convenient conversion in vitro to a fluorescent product, which can then be quantitated without the need for separation. However, more definitive and reliable information can be obtained by the measurement of urinary NMN in conjunction with one or more of the urinary pyridone turnover products (N -methyl-2-pyridone-5-carboxamide and N -methyl-4-pyridone-3-carboxamide), which can be detected and quantitated by UV absorption following high-pressure liquid chromatography. The Interdepartmental Committee on Nutrition for National Defense (USA) selected the criterion of niacin deficiency in humans as an NMN excretion rate of <5.8 tmol (0.8 mg) NMN per day in 24h urine samples. 

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