Serial lectin affinity chromatography

Serial lectin affinity chromatography Systemic Inpns erythematoses Yeast osmosensor... 

Fig. 3a. Flow diagram for the separation of the components of a complex mixture of oligosaccharides by serial lectin affinity chromatography. Depending upon the lectin adsorbant, specific oligosaccharides are either unbound (not retarded by the adsorbant), retarded (and eluted without the need of a saccharide inhibitor), or are tightly bound and then require either lOmM methyl a-D-glucopyranoside or lOOmM methyl a-D-mannopyranoside for elution. Where appropriate, each eluted peak is concentrated and the saccharide inhibitor is removed prior to application to the second affinity column. The structures of the individual oligosaccharides are shown in Fig. 3b. (Adapted from ref 288.)...

A previously-developed technique called serial lectin-affinity chromatography is available. Although the technique is almost identical to the lectin microarray in its essence, it lacks throughput and speed, because it utilizes open columns and... 

Figure 3. Serial lectin affinity chromatography of metabolically-labelled cell surface glycopeptides from a murine haemopoiet ic cell line, (a) [ H]Glucosamine (b) [ H]mannose. The cells were incubated for 48 h with either [ HJglucosamine or [ H]mannose. Washed cells were treated with tiypsin to release surface glycopeptides which were then treated with pronase. The pronase—glycopeptides were then analysed by serial lectin affinity chromatography as described in Figure Note the contrast in radiolabelling of WGA-...

Cummings, R., and Kornfeld, S. (1982). Fractionation of Asparagin-linked Oligosaccharides by Serial Lectin-agarose Affinity Chromatography, ./ Biol. Chem. 257 11235-11240. 

---