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Glycan profiling

H. van Halbeek, Glycan profiling of mammalian tissues and cells by homo- and heteronuclear nano-NMR spectroscopy, 221st ACS National Meeting, San Diego, CA, April 1-5, 2001, Abstract POLY 394. [Pg.94]

MALDl-MS Glycan Profile of Cells and Tissues SUGABASE NMR DB CCRC NMR and MS Data... [Pg.746]

Raw and aimotated MALDl-MS glycan profiles of mouse and human cells and tissues, and integration with... [Pg.746]

Glycomics Glycopeptides Glycoproteomics Mass spectrometry Nano liquid chromatography iV-glycans 0-glycans Profiling Structural analysis Tandem mass spectrometry... [Pg.2192]

CFG- Glycan Profiling MS http //www.functionalglycomics.org/glycomics/publicdata/glycoprofiling.jsp ... [Pg.2223]

MALDI is widely used for glycan profiling due to the ease of sample preparation, potential for automation, speed of data acquisition, and the simplicity of data interpretation (i.e., only singly charged ions observed)... [Pg.264]

Glycomics Glycan Profiling and In Situ Function Aided by MOE... [Pg.210]

Table 3.4 Glycan profile by HPAEC-PAD chromatography of human IgGl produced in PER.C6 cells in the three manufacturing modes. The normal ranges of the three major isoforms are presented... Table 3.4 Glycan profile by HPAEC-PAD chromatography of human IgGl produced in PER.C6 cells in the three manufacturing modes. The normal ranges of the three major isoforms are presented...
Antibodies produced on PER.C6 cells show consistent product quality, as measured by lEF, SDS-PAGE and glycan analysis in batch, fed-batch, and perfusion processes. Typical glycan profiles for antibodies produced in PER.C6 cells are similar to human serum IgG, with a ratio of GO G1 G2 of ap-... [Pg.804]

An additional type of derivative that is often used for glycan profiling and analysis is the modification of the reducing end with a chromophore, usually achieved by the formation of a Schiff base with an aromatic amine, and subsequent reduction with sodium borohydride to stabilize the initially formed product by its conversion to a saturated amine. This procedure was initially developed for HPLC analysis with ultraviolet detection, but it is also appropriate for MS analysis because the substituted amino group represents a site for charge localization that simplifies the fragmentation pattern and also provides the opportunity to introduce a stable isotope label for quantitative purposes (54). [Pg.50]

MALDI-TOF MS is also very useful for analysis of glycoproteins, particularly when an infrared (2.94 pm) laser is employed (62). For instance, for ai-acid glycoprotein with heterogeneous glycan profiles, the MALDI-TOF mass spectrum shows a broader peak than would be observed for a typical unmodified protein in the same mass range this broadness reflects the glycoform heterogeneity. [Pg.51]

See other pages where Glycan profiling is mentioned: [Pg.103]    [Pg.248]    [Pg.155]    [Pg.2]    [Pg.225]    [Pg.263]    [Pg.75]    [Pg.597]    [Pg.742]    [Pg.745]    [Pg.748]    [Pg.1883]    [Pg.2117]    [Pg.2195]    [Pg.2202]    [Pg.2202]    [Pg.2221]    [Pg.2228]    [Pg.185]    [Pg.262]    [Pg.247]    [Pg.248]    [Pg.266]    [Pg.267]    [Pg.268]    [Pg.811]    [Pg.1860]    [Pg.96]    [Pg.384]    [Pg.34]    [Pg.51]    [Pg.53]    [Pg.367]    [Pg.670]    [Pg.268]    [Pg.268]   
See also in sourсe #XX -- [ Pg.861 ]

See also in sourсe #XX -- [ Pg.45 , Pg.46 , Pg.47 , Pg.48 , Pg.49 , Pg.50 , Pg.51 ]

See also in sourсe #XX -- [ Pg.239 , Pg.245 ]



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