Lectins carbohydrate specificity

Lis, H. Sharon, N., Lectins Carbohydrate-specific proteins that mediate cellular recognition. Chem. Rev. 1998, 98, 637-674. 

Sharon, N. Lectins carbohydrate-specific reagents and biological recognition molecules. J. Biol. Chem. 2007,282, 2753-2764. 

This rapidly developing field offers tremendous scope for preventing bacterial colonization and infections in humans. The lectin/carbohydrate specific interactions are potentially useful for a wide range of industrial applications since the preparation of plant lectins are... 

Lis, H Sharon, N. Lectins Carbohydrate-Specific Proteins That Mediate Cellular Recognition. Chew Rev., 1998, 98(2), 637-74. 

Specificity and affinity studies in lectin/carbohydrate interactions 356... 

Specific detection of oligosaccharides is possible using lectins, carbohydrate-binding proteins from very different sources. A se-... 

Lectin-Carbohydrate Interactions Are Very Strong and Highly Specific... 

Protein fractions having lectin activity have been extracted from mitochondria, plasma, and Golgi membranes, and from the endoplasmic reticulum of mung-bean hypocotyls, as well as from total-membrane fractions from a variety of plant tissues.257 The carbohydrate specificity of the lectin fractions differs with the membrane type. It is conceivable that, in addition to cell-wall lectin-membrane interactions, there may also be membrane lectin-cell wall, noncovalent bonds. 

Lectins are carbohydrate-binding proteins that have been obtained mainly from plants,1128,138 although lectin-like proteins have also been found in micro-organisms and animals,139 including mammals.140 141 Some of the properties of the lectins, and their utility as carbohydrate-specific reagents have been reviewed.1128,138,138... 

Many lectins combine preferentially with a single sugar structure, for example g-galactose or L-fucose. For some lectins the specificity is broader and includes several closely related sugars, e.g., D-man-nose, g-glucose and g-arabinose other lectins-interact only with complex carbohydrate structures such as those that occur in glycoproteins, glycolipids,... 

Antibodies directed against recombinant erythropoietin have been obtained from rabbits immunized with the hormone and Freunds complete adjuvant [64], Two sets of antibodies are present in the serum of rabbits that have been immunized, Fig. (3C). The results of oxidation of the erythropoietin with periodate, Fig. (25A), reaction of the antibodies with lectins of known carbohydrate specificity, Fig. (25B) and inhibition of the antibodies with the structural monosaccharide residues of the hormone, Fig. (26), have established the types of antibodies to be anti-carbohydrate antibodies and the immunodeterminants for these antibodies are oligosaccharides with the following structure N-acetyl neuraminyl a(2->3)... 

In their interaction with saccharides, lectins serve as models for carbohydrate-specific antibodies, with the important advantage that it is possible to purify lectins in gram quantities. Furthermore, lectin combining-sites appear to be homogeneous and noninteracting, in contrast to those of immune antibodies. 

A thorough study of pea-lectin, carbohydrate-binding specificity was conducted by Van Wauwe and coworkers211 (see Table V). By hapten-... 

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