LC/NMR liquid chromatography

LC-MS/MS Liquid chromatography coupled with tandem mass spectrometry LC-NMR Liquid chromatography coupled with nuclear magnetic resonance TLC Thin-layer chromatography... [Pg.100]

LC-MS (liquid chromatography-mass detector) and LC-NMR (liquid chromatography-nuclear magnetic resonance spectroscopy). [Pg.288]

LC/NMR Liquid Chromatography/Nuclear Magnetic Resonance Spectroscopy... [Pg.349]

FT, Fourier transformation LC-NMR, liquid chromatography and nuclear magnetic resonance. [Pg.1]

Methods in the analysis of drug impurities (e.g., ultraviolet, UV Fourier transform infrared, FT-IR nuclear magnetic resonance, NMR mass spectrometry, MS) are used to separate, identify, and quantify impurities, as well as establish their structure. Currently the most efficient methods seem to be combined techniques such as GC-MS, LC-MS, liquid chromatography-diode-array detection-mass spectrometry (LC-DAD-MS), LC-NMR, LC-DAD-NMR-MS, etc. [18-20]. [Pg.189]

LC-MS-NMR liquid chromatography-mass spectrometry-nuclear magnetic resonance... [Pg.588]

The explanation of the biosynthetic pathway may also be possible through molecular biology techniques that use mutants. The use of tandem analytical instrumentation (e.g., GC/MS (gas chromatography/mass spectrometry), NMR/MS, LC/MS (liquid chromatography/mass spectrometry)) has improved the identifications of primary and secondary metabolites. [Pg.4]

Several analytical tools coupled to mass spectrometers as GC-MS (gas chromatography) and LC-MS (liquid chromatography), including ICP-MS (inductively coupled argon plasma), SCF-MS (supercritical fluid), NMR-MS (nuclear magnetic resonance), and IR-MS (infrared-MS), are routinely used to determine the phenolic acids. With the development of soft ionization techniques, fast atom bombardment (FAB) [19] and plasma desorption (PD) [20], followed by the electrospray ionization (ESI) [21], have been used for phenolic acid analyses. [Pg.4563]

Detailed kinetic studies, product studies, NMR spectroscopic analyses, LC-MS (liquid chromatography-mass spectrometry) chromatography, and Cl and deuterium tracer studies have shown that the homogeneous hydrolysis of 2-chloroethyl ethyl sulfide in water and in 1 1 acetone/water at concentrations above 10 M occurs by the mechanism in Scheme 10. °... [Pg.353]

As liquid chromatography plays a dominant role in chemical separations, advancements in the field of LC-NMR and the availability of commercial LC-NMR instrumentation in several formats has contributed to the widespread acceptance of hyphenated NMR techniques. The different methods for sampling and data acquisition, as well as selected applications will be discussed in this section. LC-NMR has found a wide range of applications including structure elucidation of natural products, studies of drug metabolism, transformation of environmental contaminants, structure determination of pharmaceutical impurities, and analysis of biofiuids such as urine and blood plasma. Readers interested in an in-depth treatment of this topic are referred to the recent book on this subject [25]. [Pg.363]

Hyphenated analytical techniques such as LC-MS, which combines liquid chromatography and mass spectrometry, are well-developed laboratory tools that are widely used in the pharmaceutical industry. Eor some compounds, mass spectrometry alone is insufficient for complete structural elucidation of unknown compounds nuclear magnetic resonance spectroscopy (NMR) can help elucidate the structure of these compounds (see Chapter 20). Traditionally, NMR experiments are performed on more or less pure samples, in which the signals of a single component dominate. Therefore, the structural analysis of individual components of complex mixtures is normally time-consuming and less cost-effective. The... [Pg.14]

Important progress in terms of higher throughput in ADME/PK work was realized recently by wider use of liquid chromatography/mass spectrometry (LC/MS), which has now become a standard analytical tool [26]. Flow NMR spectroscopy has become a routine method to resolve and identify mixtures of compounds and has found applications in drug metabolism and toxicology studies [27]. [Pg.137]