Matrix-assisted laser desorption quantitative

May, L.A. et al.. Detection and quantitation of curcumin in mouse lung cell cultures by matrix-assisted laser desorption ionization time of flight mass spectrometry. Anal. Biochem., 337, 62, 2005. 

Liquid chromatography/mass spectrometry Lower limit of detection Limit of detection Limit of quantitation Florseshoe crab hemocyanin Liquid scintillation counting Matrix-assisted laser desorption/ ionization mass spectrometry m -Maleimidobenzoy 1-A -Hydroxysuccinimide 1 -Cyclohexyl-3-(2-Morptiolino-ethyl)carbodiimide rnetlio-/ -Toluenesulfonate (same as CDI)... 

In a separate study, a protocol for Matrix-assisted laser desorption-ionization (MALDI) imaging mass spectrometry (IMS) has been proposed.18 This IMS technique provides a new approach to visualize spatial distribution of thousands of molecular species, including peptides, proteins, and their metabolites in two- or three-dimensional levels. This approach may also provide a straightforward method of determining the tissue distribution of multiple peptides or proteins in a quantitative manner.18 Chu et al.19 reported a nondestructive molecular extraction method to obtain proteins from a single FFPE or frozen tissue section, without destroying the tissue morphology, such... 

O. A. Mirgorodskaya, Y. P. Kozmin, M. I. Titov, R. Kbmer, C. P. Sonksen, and P. Roepstorff. Quantitation of Peptides and Proteins by Matrix-Assisted Laser Desorption/ Ionization Mass Spectrometry Using lsO-Labeled Internal Standards. Rapid Commun. Mass Spectrom., 14(2000) 1226-1232. 

This proposal describes the development of a new, systematic approach for qualitatively and quantitatively studying surface-biomolecule interactions by matrix-assisted laser desorption ionization (MALDl) mass spectrometry (MS). This methodology is being developed because of the profound importance that surface-biomolecule interactions play in applications where biomaterials come into contact with complex biological fluids, it can readily be shown that undesired reactions occurring in response to surface-biomolecule contact (protein adsorption, biofouling, immune response activation, etc.) lead to enormous economic and human costs. Thus, the development of analytical methodologies that allow for efficient assessment of the properties of new biomaterials and/or the study of detailed fundamental processes initiated upon surface-biomolecule contact are of critical value ... 

Zabet-Moghaddam, M., Heinzle, E. and Tholey, A., Qualitative and quantitative analysis of low molecular weight compounds by ultraviolet matrix-assisted laser desorption/ionization mass spectrometry using ionic liquid matrices. Rapid Commun. Mass Spec., 18,141, 2004. 

Gusev, A. I. et al.. Direct quantitative analysis of peptides using matrix assisted laser desorption ionization. Anal. Bioanal. Chem., 354, 455, 1996. 

Duncan MYV, Matanovic G, Cerpa-Poljak A (1993) Quantitative analysis of low molecular weight compounds of biological interest by matrix-assisted laser desorption ionization. Rapid Commun Mass Spectrom 7 1090-1094... 

Hatsis, P., Brombacher, S., Corr, J., Kovarik, P., and Volmer, D. A. (2003). Quantitative analysis of small pharmaceutical drugs using a high repetition rate laser matrix-assisted laser/ desorption ionization source. Rapid Commun. Mass Spectrom. 17 2303-2309. 

Jespersen, S., Niessen, W. M. A., Tjaden, U. R., and van der Greef, J. (1995). Quantitative bioanalysis using matrix-assisted laser desorption/ionization mass spectrometry. J. Mass Spectrom. 30 357-364. 

Knochenmuss, R. (2003). A quantitative model of ultraviolet matrix-assisted laser desorption/ ionization including analyte ion generation. Anal. Chem. 75 2199 -2207. 

Muddiman, D. C., Gusev, A. I., and Hercules, D. M. (1996). Application of secondary ion and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry for the quantitative analysis of biological molecules. Mass Spectrom. Rev. 14 383-429. 

Kang, M. J., Tholey, A., and Heinzle, E. (2000). Quantitation of low molecular mass substrates and products of enzyme catalyzed reactions using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom. 14 1972-1978. 

T. Fujiwaki, M. Tasaka, N. Takahashi, H. Kobayashi, Y. Murakami, T. Shimada, and S. Yamaguchi, Quantitative evaluation of sphingolipids using delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometry with sphingosylphosphorylcholine as an internal standard. Practical application to cardiac valves from a patient with Fabry disease, J. Chromatogr. B. 832 (2006) 97-102. 

Bilati U, Pasquarello C, Corthals GL, et al. Matrix-assisted laser desorption/ioniza-tion time-of-flight mass spectrometry for quantitation and molecular stability assessment of insulin entrapped within PLGAnanoparticles./. Pharm. Sci. (2005) 94 688-694. 

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