IP-RP-HPLC

Direct and derivative spectrophotometric and IP-RP-HPLC methods were applied to identify and determine synthetic dyes and follow their degradation processes. " The dyes considered were Tartrazine (E 102), Quinoline Yellow (E 104), Sunset Yellow (E 110), Carmosine (E 122), Amaranth (E 123), New Coccine (E 124), Patent Blue Violet (E 131), and Brillant Blue ECE (E 133). All are considered representative additives for soft drinks. 

Gianotti, V. et al., Chemometrically assisted development of IP/RP/HPLC and spectrophotometric methods for the identification and determination of synthetic dyes in commercial soft drinks, J. Liq. Chromatogr. Rel. TechnoL, 28, 923, 2005. 

Desalted peak fractions were analyzed by both IP-RP-HPLC and RP-HPLC (derivatized with FMOC-Cl) to investigate the presence of pyridinoline- and lysinonorleucine type cross-links, respectively. Two fractions. 111 and V-1-1, were found to contain the cross-links HP and DHLNL, respectively. Additional analyses by cation exchange HPLC showed TV and V-2 as closely eluting peaks and VI as a mixture of lysi-noalanine and an unidentified amino acid (fig. 3a). 

S/DVB Thermal, AIBN 1-Decanol/THF 200 pm LD. capillary columns, IP-RP-HPLC of nucleic acids and RP-HPLC of proteins and peptides [49,137]... 

Styrene monoliths have been prepared by thermally (AIBN or benzoyl peroxide) initiated copolymerization of styrene and divinylbenzene to result mechanically stable, hydrophobic column supports for RPC as well as IP-RP-HPLC and CEC application [24,49,134-140]. 

Samples used to demonstrate this method were soil fulvic acid (SFA) and water fulvic acid (WFA), both well-characterized materials obtained from Dr. James H. Weber at the University of New Hampshire (19). Figure 1 shows that the chromatographic method resulted in four fractions separated for the SFA. The use of IP-RP-HPLC with the biological buffer MES resulted in sufficient separation to eliminate the need for gradients as have been used in previous studies (13-17). Simultaneous collection of UV (254 nm) and fluorescence (A citation = 332 nm and Remission = 42 nm) data showed similar chromatograms with peaks at the same retention times except in the case of the more non-polar (later-eluting) fractions which did not exhibit measurable fluorescence. This result is similar to that reported by Lombardi et al. (75) for marine DOM. Figure 2 shows a very similar separation for WFA. 

Figure 6 High-resoiution capiiiary iP-RP-HPLC separation of phosphoryiated oiigodeoxynucieotide iadders in a monoiithic capiiiary coiumn. Coiumn, continuous PS/DVB, 60 x 0.20mm i.d. mobiie phase (A) lOOmmoii TEAA, pH 6.97 (B) lOOmmoii TEAA, pH 6.97, 20% acetonitriie iinear gradient, (a) 15-45% B in 3.5 min, 45-55% B in 2.5min, 55-65% B in 4.0 min fiow-rate, 2.5 pi min k temperature, 50°C detection, UV, 254 nm sampie, p(dA),2-i8. P dT)i2-3o. 40-98fmoi of each oiigodeoxynucieotide. (Reprinted with permission from Premstaiier et al. (2000) Analytical Chemistry 72 4388 2003 American Chemicai Society.)...

D. P. Hornby, High throughput analysis of nucleic acid modification reactions using IP RP HPLC, Amd. Biochem. 301, 290, 2002. 

Figure 16 IP-RP-HPLC separation of an oligodeoxynucleotide (dT)i2-is on a ROMP-derived NBE-based monolith (3 x 60 mm). Mobile phase 100 mmol f triethylammonium acetate at pH 7.0 linear gradient, 11-16% acetonitrile in 10min flow rate, 2mlmin 7"=20 C detection, UV 264 nm sample (dT)i2-i8, 0.1 pg of each oligodeoxynucleotide.

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