Ion trap array

Tabert, A. M., Griep-Raming, J., Guymon, A. J., and Cooks, R. G. (2003). High-throughput miniature cylindrical ion trap array mass spectrometer. Anal. Chem. 75, 5656—5664. 

Figure 10 A schematic represerttation of two of the four parallel analysis channels in the 2 x 2 array of mini-CITs. (Reproduced with permission from Tabert AM, Griep-Raming J, Guymon AJ, and Cooks RG (2003) High-throughput miniature cylindrical ion trap array mass, spectrometer. Analytical Chemistry 75 5656-5664. Copyright 2003, by American Chemical Society.)...

Miniature cylindrical ion trap array An approach to the rapid screening of large numbers of samples in the areas of proteomics, industrial process monitoring, and metabolomics is to use a multiplexed inlet system with a multiplexed mass spectrometer having an equal number of parallel sample channels. To this end, a high-throughput mini-CIT array mass spectrometer has been developed and tested. 

Badman ER and Cooks RG (2000) A parallel miniature cylindrical ion trap array. Analytical Chemistry 72 3291-3297. 

The development of the DIT has been pursued by Shimadzu and has reached a quite impressive level. Recently, an array of DITs, termed ion trap array (ITA), has been developed allowing multiple steps of isolation, activation, or scanning in parallel [164]. 

Other types of mass spectrometer may use point, array, or both types of collector. The time-of-flight (TOF) instrument uses a special multichannel plate collector an ion trap can record ion arrivals either sequentially in time or all at once a Fourier-transform ion cyclotron resonance (FTICR) instrument can record ion arrivals in either time or frequency domains which are interconvertible (by the Fourier-transform technique). 

There are two common occasions when rapid measurement is preferable. The first is with ionization sources using laser desorption or radionuclides. A pulse of ions is produced in a very short interval of time, often of the order of a few nanoseconds. If the mass spectrometer takes 1 sec to attempt to scan the range of ions produced, then clearly there will be no ions left by the time the scan has completed more than a few nanoseconds (ion traps excluded). If a point ion detector were to be used for this type of pulsed ionization, then after the beginning of the scan no more ions would reach the collector because there would not be any left The array collector overcomes this difficulty by detecting the ions produced all at the same instant. 

Other types of mass spectrometer can use point, array, or both types of ion detection. Ion trap mass spectrometers can detect ions sequentially or simultaneously and in some cases, as with ion cyclotron resonance (ICR), may not use a formal electron multiplier type of ion collector at all the ions can be detected by their different electric field frequencies in flight. 

Flow injection Chemical ionisation (Cl) Quadrupole ion trap (QITMS) Diode array detector... 

HPLC is a universal separation technique that is capable of separating both volatiles and non-volatiles without the need for derivatization. We are developing methods that employ both on-line photodiode array (PDA) detection and mass selective detection, HPLC/PDA/MS. This approach also utilizes an ion-trap mass... 

Multiple mass analyzers exist that can perform tandem mass spectrometry. Some use a tandem-in-space configuration, such as the triple quadrupole mass analyzers illustrated (Fig.3.9). Others use a tandem-in-time configuration and include instruments such as ion-traps (ITMS) and Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS or FTMS). A triple quadrupole mass spectrometer can only perform the tandem process once for an isolated precursor ion (e.g., MS/MS), but trapping or tandem-in-time instruments can perform repetitive tandem mass spectrometry (MS ), thus adding n 1 degrees of structural characterization and elucidation. When an ion-trap is combined with HPLC and photodiode array detection, the net result is a profiling tool that is a powerful tool for both metabolite profiling and metabolite identification. 

C. Baiocchi, M.C. Brussino, E. Pramauro, A.B. Prevot, L. Palmisano and G. Marci, Charac-terizaton of methyl orange and its photocatalytic degradation products by HPLC/UV-VIS diode array and atmospheric pressure ionization quadrupole ion trap mass spectrometry. Int. J. Mass Spectr., 214 (2002) 247-256. 

HPLC - Beckman 125 binary gradient pumps, 168 diode-array detector, 507 autosampler MS - Ion-trap mass spectrometer Finnigan LCQ equipped by APCI (atmospheric pressure chemical ionization), data analyzed in negative mode, spectra confirming found compounds were obtained from tandem mass spectromectry (MS/MS). 

Liquid chromatography/mass spectrometry analyses were performed with an ion trap mass spectrometer (LCQ, Thermo Fisher Scientific Inc., MA) equipped with an HPLC system (Agilent, CA Model 1100) connected with a diode-array detector (DAD, G1315A). The sample solution (1-5 p,L) was applied on an Inertsil ODS-3 column (2.1 x 150 mm, 3 p,m, GL... 

Elemental mass spectrometry has undergone a major expansion in the past 15-20 years. Many new a, elopments in sample introduction systems, ionization sources, and mass analyzers have been realized. A vast array of hybrid combinations of these has resulted from specific analytical needs such as improved detection limits, precision, accuracy, elemental coverage, ease of use, throughput, and sample size. As can be seen from most of the other chapters in this volume, however, the mass analyzers used to date have primarily been magnetic sector and quadrupole mass spectrometers. Ion trapping devices, be they quadrupole ion (Paul) [1] traps or Fourier transform ion cyclotron resonance (Penning) traps, have been used quite sparingly and most work to date has concentrated on proof of principal experiments rather that actual applications. 

Arapitsas P. Sjoberg P.J.R. Turner C. 2008. Characterisation of anthocyanins in red cabbage using high resolution liquid chromatography coupled with photodiode array detection and electrospray ionization-linear ion trap mass spectrometry. Food Chem. 109 219-226. 

Mertz, C. Cheynier, V. Brat, P. 2007. Analysis of phenolic compounds in two blackberry species (Rubus glaucus and Rubus adenotrichus) by high-performance liquid chromatography with diode array detection and electrospray ion trap mass spectrometry. J. Agric. Food Chem. 55 8616-8624. 

This is considered the method of choice for the analysis of flavones and flavonols due the high resolution of the chromatographic separations and the sensitivity of the detection methods that include UV, fluorescence, electrochemical, and MS detectors. The best combination is the detection system that links on-line UV detection with a photodiode array detector (PDA) that allows the registration of the UV spectra of the eluting compounds, with an MS/MS detector as an ESI ion trap that allows the isolation and fractionation of specific ions, even if they coelute under the same chromatographic peak. 

---