Lipids in tissues

Increased synthesis of lipid or uptake. Increased synthesis of lipid may be the cause of fatty liver after hydrazine administration as this compound increases the activity of the enzyme involved in the synthesis of diglycerides. Hydrazine also depletes ATP and, however, inhibits protein synthesis. Large doses of ethanol will cause fatty liver in humans, and it is believed that this is partly due to an increase in fatty acid synthesis. This is a result of an increase in the NADH/NAD"1" ratio and therefore of the synthesis of triglycerides. Changes in the mobilization of lipids in tissues followed by uptake into the liver can also be another cause of steatosis. 

Lipid Melting Temperatures Membrane lipids in tissue samples obtained from different parts of the leg of a reindeer have different fatty acid compositions. Membrane lipids from tissue near the hooves contain a larger proportion of unsaturated fatty acids than those from tissue in the upper leg. What is the significance of this observation ... 

Bolgova, O.M., Lizenko, E.I. and Yarzhombek, A.A. (1985a). Qualitative composition of lipids in tissues of one year carp after wintering (In Russian). Rybnoye Khozyaistvo 1985 (12), 27-29. 

The concentrations of PFAs in environments have increased sharply over time (Holmstrom et al., 2005). The sorption/accumulation and fate of PFAs differ from classical chlorinated and brominated POPs in that perfluorinated molecules have surfactant characteristics (i.e., having polar and nonpolar domains) and oleophobic properties. Thus, PFAs is likely to bind to serum protein rather than lipid in tissues (Jones et al., 2003). 

Notwithstanding the possible association of the aforementioned nonlipid components with lipids in tissue, the fact remains that if the objective is to obtain a clean, highly purified preparation of phospholipids for further chemical study, for example, then these substances must be removed. 

In Lipid (%) In Tissue (%) Ester Iodine Value In Lipid (%) In Tissue (%) Ester Iodine Value... 

B16. Buck, B. C., Distribution of acid mucopolysaccharides and lipids in tissues of cholesterol-fed rabbits. A.M.A. Arch. Pathol. 58, 576-587 (1954). 

Sokolova, G.P. 1982. Assay of a content and determination of specific radioactivity of total lipids in tissues. In Methods of Biochemical Analysis A Manual (Ed. by M.l. Prokhorova), pp. 55—58. Leningrad Leningrad University Press (in Russian). 

When tissue samples are ready to be analyzed, they are dehydrated in a vacuum desiccator at room temperature to remove moisture and avoid lateral migration of analytes before application of MALDI matrix. Traditional low-pressure ( 10 torr) MALDI requires samples to be dried completely ( 2 h) before exposure to vacuum conditions. This prohibits the analysis of freshly cut tissue and reduces sample throughput. In addition, low-pressure MALDI has been shown to produce in-source fragmentation of lipids in tissue, which makes... 

Sddergren, E, Vessby, B and Basu, S (2000) Radioimmunological measurement of Fj-isoprostanes after hydrolysis of lipids in tissues. Frost Leuk Ess Fatty Acids, 63,149-152. 

In addition to synthesis in loco, potential sources of cholesterol and other lipids in tissues where red cell destruction occurs, are lipids of erythrocytes (Fredrickson 1966), but the quantitative aspects of this process are not known. 

MALDI-MS has already been successfully applied for direct analysis of lipids in tissue samples. This topic is extensive described in Chapter 12. 

A drawback of DESI-IMS analysis of lipids in tissue sections in comparison to that of MALDI or SIMS is its tissue damage to a much larger degree. This consequence makes the tissue samples after DESI analysis difficult to acquire the complementary information such as histological data for direct comparison between data sets. To resolve this issue, Eberlin et al. developed a method [29,104] by using binary solvent... 

Vnlt Fractional volume of neutral nonpolar lipids in tissue ... 

Yellow deposits of lipids in tissues of the body, including the skin. They can be found in hyperlipidaemic conditions. 

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