Column ion exchange chromatography

Leucrose, 6-0-(a-D-glucopyranosyl)-P-D-fmctopyranose [7158-70-5] is synthesized from sucrose usiag a dextranase enzyme from l euconostoc mesenteriodes and a small proportion of fmctose (2%). Pfeifer Langen of Germany have developed a production process for leucrose that iavolves extraction of the enzyme, treatment with 65% aqueous solution of sucrose and fmctose (1 2 wt/wt) at 25°C, separation of the product from fmctose by ion-exchange column chromatography, and crystallization. The product has not yet been launched on the market as of this writing (1996). 

Mercer, D. W. Separation of tissue and serim creatine kinase isoenzymes by ion-exchange column chromatography. Clin. Chem. (1974), 20, 36-40. 

For wheat grain, extract the concentrate three times with 10 mL of chloroform-methanol (3 1, v/v). For the soil sample, extract the aqueous layer after washing with n-hexane twice with 60 mL of chloroform-methanol (3 1, v/v). Dry the chloroform-methanol layer with anhydrous sodium sulfate [for wheat grain, use a small amount (about 8 g) of anhydrous sodium sulfate] and collect the dried solution in a 200-mL round-bottom flask. Evaporate the solvent under reduced pressure and proceed to ion-exchange column chromatography. 

McCartney, J. E., Rapid purification of a recombinant protein using tandem radial flow ion-exchange column chromatography, BioTechniques, 11,648,1991. 

Garon, C. F. and Peterson, L. L., An improved method for the isolation of supercoiled DNA molecules using ion-exchange column chromatography, Gene Anal. Technol., 4, 5, 1987. 

Liquid Chromatography. - Diasteriomeric phosphonodipeptides have been separated by ion exchange column chromatography.267 H.p.l.c. has been used for the analysis of a variety of biologically active phosphorus compounds, such as aminoacid phosphate esters,26 phosphinothrycin,269 inositol triphosphate,270 fructose diphosphate,271 pyridoxal phosphate,272 and ATP.273... 

Tasaka, K Kobayashi, M Tanaka, T and Inagaki, C. (1984) Rapid purification of monoclonal antibody in ascites by high performance ion exchange column chromatography for diminishing non-specific staining. Acta Histochem. Cytochem. 17, 283-286. 

Levison PR. Large-scale ion exchange column chromatography of proteins Comparison of different formats. Journal of Chromatography B 2003 790 17-33. 

The isolation of an individual glycosyl ester of a nucleoside pyrophosphate is a rather difficult task. Ion-exchange column-chromatography and preparative, paper-chromatography have been the main methods used for solving problems in separation. 

Column chromatography yields quantitative results even for constituents present in only small amounts (with the exception of tryptophan, methionine, and glutamine, in which cases the recovery is not complete see below, (b) Dowex 50-X4 columns). It lends itself also to extraction on a preparative scale for further analysis of a particular constituent it does not require previous desalting of urine. The major inconvenience of ion exchange column chromatography for routine analyses is that the technique is elaborate and time consuming, and requires experienced personnel and expensive equipment. Recent developments, however, have allowed for considerable reduction in time consumption, and the latest completely automatic devices open the way to serial analyses. 

In early infancy the situation is known to be quite different from that among older children. Simon noticed in 1911 that the newborn infant excreted 10 % of its total nitrogen output in the form of amino nitrogen, whereas in adults it drops to 2 % (S15). This was confirmed in later years (B4, Cll, G5) and extended to the study of individual amino acid excretion by microbiological methods (S10, Sll), by paper chromatography (S20, S21), and finally by ion exchange column chromatography (D28, D30) at the time Moore and Stein s first method of 1951 was still the only one available. 

Ion-exchange column chromatography Membrane-based ion exchange chromatography High volumetric efficiency... 

The colonial sea anemone Anthopleura elegantissima responds with characteristic contraction to a pheromone released by wounded conspecifics. This alarm response is highly characteristic, includes rapid bending and shortening of the tentacles and depression of the oral disk. In 1975, by extensive ion exchange column chromatography, (3-carboxy-2,3-dihydroxy-A(A(Af-trimethyl)-l-propanaminium chloride (50) was isolated as a pure crystalline substance.116 It showed alarm pheromone activity with a median concentration of 0.35 nmol 1 1 and was named anthopleurine. Comparison of spectral data between natural and synthetic compounds revealed that anthopleurine had a structure of 4-amino-4-deoxy-L-threonic acid betaine hydrochloride.117... 

The hydroboration of ( )-baikiain (4,5-dehydro-( )-pipecolinic acid) via its JV-benzyloxycarbonyl methyl ester derivative (134) led, after oxidation and removal of the protecting groups, to 72% trans-5-hydroxy-( + )-pipecolinic acid (136) and 28% (raw.s-4-hydroxy-( + )-pipecolinic acid (135), separable by preparative ion-exchange column chromatography.118... 

It is the author s opinion that ion-exchange column chromatography,... 

Hydrochlorothiazide can be separated from reserpine and hydralazine using ion-exchange column chromatography. A solution of known concentration is then subjected to analysis by UV spectroscopy and compared to a standard solution. The quantity of hydrochlorothiazide present in the sample is then determined. 

Chromatography. Separations by ion exchange column chromatography were performed according to ASTM methods (10,11). The Technicon AutoAnalyzer (Bran and Luebbe) (12,13) was employed for analysis of the eluent from ASTM method D 2761 (11-13). ASTM method D 501 was followed as written, using the ammonium molybdate colorimetric analysis of the hydrolyzed fractions. 

LC-separation of low molecular-weight constituents of nucleic acids and intact nucleic acids was reviewed by Zadrazil [358,359], Brown [360] described an enzyme peak shift method verifying peak identities of nucleotides, Singhal [361] reviewed separation and analysis of nucleic acids and their constituents by ion-exclusion and ion exchange column chromatography, and Brown [31,362] summarized the latest developments and state-of-art in HPLC of nucleic acid constituents. Plunkett [363] dealt with the use of HPLC in research of purine nucleoside analogs. 

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