Creatine kinase isoenzymes

Wallimann, T., Wyss. M., Brdicza, D., Nicolay, K.. Eppenberger, H.M. (1992). Intracellular compartmentation. structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy demands The phosphocreatine circuit for cellular energy homeostasis. Biochem. J. 281,21-40. 

Mercer, D. W. Separation of tissue and serim creatine kinase isoenzymes by ion-exchange column chromatography. Clin. Chem. (1974), 20, 36-40. 

Henry, P. D. Roberts, R. and Sobel, B. E. Rapid separation of plasma creatine kinase isoenzymes by batch absorption on glass beads. Clin. Chem. (1975), 21, 884-849. 

Jockers, W. Wretouy, E. Pfleiderer, 6. Quantitation of creatine kinase isoenzymes in human tissues and sera by an immunological method. Clin. Chim. Acta. (1975),... 

As an example, consider the separation of the creatine kinase isoenzymes, MM, MB, and BB. Mercer has used classical ion-exchange chromatography (DEAE - Sephadex - A50) for the resolution of these three isoenzymes (44) To speed up the separation and ultimately to allow an automated analysis,... 

Figure 6, High pressure liquid chromatogram of creatine kinase isoenzymes. First peak, MM second peak, BB. Conditions 50 cm X 4.8 mm (i.d.) column with yydac porous layer bead anion exchange mobile phase, step gradient Solvent A, 10 mmol/liter Tris buffer, pH 8.3 solvent B, 10 mmol/liter Tris buffer, pH 7.0,0.5 mol KCl flow rate, 2 ml/min detection, collected fractions assayed (45).

Wyss, M. Schlegel, J. James, P. Eppenberger, H.M. Wallimann, T. Mitochondrial creatine kinase from chicken brain. Purification, biophysical characterization, and generation of heterodimeric and heterooctameric molecules with subunits of other creatine kinase isoenzymes. J. Biol. Chem., 265, 15900-15908 (1990)... 

Schlattner, U. Eder, M. Bolder, M. Khuchua, Z.A. Strauss, A.W. Walli-mann, T. Divergent enzyme kinetics and structural properties of the two human mitochondrial creatine kinase isoenzymes. Biol. Chem., 381, 1063-1070 (2000)... 

Mourad-Terzian, T. Steghens, J.P. Min, K.L. Collombel, C. Bozon, D. Creatine kinase isoenzymes specificities histidine 65 in human CK-BB, a role in protein stability, not in catalysis. FEBS Lett., 475, 22-26 (2000)... 

Subunit structure and electrophoretic mobility and enzyme activity of creatine kinase isoenzymes. 

Reference Intervals for Cardiac Troponins and Creatine Kinase Isoenzymes... 

Sket, D., Brzin, M., Vreca, I. (1989). Effect of HI-6 and diazepam on the increase of creatine kinase isoenzymes activity in plasma of atropinized, soman-poisoned rats. Acta Pharm, Jugoslav. 39 151-9. 

VoL LVn [6]. Determination of Creatine Kinase Isoenzymes in Human Serum by an Immunological Method Using Purified Firefly Luciferase. A. Lundin. 

Chazan R, Tadeusiak W, Jaworski A, Droszcz W. Creatine kinase (CK) and creatine kinase isoenzyme (CK-MB) activity in serum before and after intravenous salbutamol administration of patients with bronchial asthma. Int J Clin Pharmacol Ther Toxicol 1992 30(10) 371-3. 

ST92 Lianidou, E.S., Christopoulos, T.K. and Diamandis, E.P. (1990). Assay of creatine kinase isoenzyme MB in serum with time-resolved immunofluorom-etry. Clin. Chem. 36, 1679-1683. 

The analytical performance of the methods used for many clinical tests has improved dramatically over the past 4 decades. However, a highly accurate and precise test may provide less clinicai information than a different test that is less accurate and less precise. For example, a test for total creatine Idnase is often more accurate and precise than one for creatine kinase isoenzymes, yet knowledge of the total enzyme activity is of less value in the diagnosis of cardiac damage. How does one evaluate the information content of a test What procedure should one use to decide among different tests based on their disease discrimination ability This chapter discusses these and other nonanalytical aspects of test performance that affect a test s overall medical usefulness. 

Panteghini M. Serum isoforms of creatine kinase isoenzymes. Clin Biochem 1988 21 211-8. 

The ProstAsure index, developed by Zhang, Stamey, and Chan, was designed to increase the sensitivity and specificity of cancer detection, while maintaining a reasonable false positive detection rate. The index is derived firom several values, including age, total PSA, creatine kinase isoenzymes, and prostatic acid phosphatase, which are input... 

Gerhardt W, Katus H, RavkHde J, Hamm C, Jorgensen PJ, Peheim E, et al. S-Troponin T in suspected ischemic myocardial injury compared with mass catalytic concentrations of S-creatine kinase isoenzyme MB. Clin Chem 1991 37 1405-11. 

Lang H. Creatine kinase isoenzymes. New York Springer-Verlag, 1981. 

Sharkey SW, Murakami MA, Smith SA, Apple FS. Canine myocardial creatine kinase isoenzymes after chronic coronary artery occlusion. Circulation 1991 84 333-40. 

Wu AHB, Schwartz JG. Update on creatine kinase isoenzyme assays. Diagn Clin Test 1989 27 16-9. 

K18. Kudirka, P. J., Busby, M. G., Carey, R. N., and Toren, E. C., Jr., Separation of creatine kinase isoenzymes by high-pressure liquid chromatography. Clin. Chem. 21, 450-452 (1975). 

U. Wuerzburg, Measurement of Creatine Kinase Isoenzyme Activity by Immunological Methods, in Creatine Kinase Isoenzymes Pathophysiology and Clinical Application (ed. H. Lang), Springer-Verlag, New York, 1981, p. 49. 

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