Inulin acetate

Inulin Acetate. Stir 100 g. of inulin in 1,000 ml. of pyridine at 80° for forty-five minutes. Cool, while continuing the stirring, and to the clear solution add dropwise 180 ml. of acetic anhydride. After six hours more of stirring, pour the clear solution into 10 liters of water. The inulin acetate separates as a white solid. Filter and wash repeatedly with distilled water to remove the pyridine and acetic anhydride. Purify the dried crude product by dissolving in ten times its weight of hot methyl alcohol and filtering. Inulin acetate separates from the cold solution as a fine white powder [ajj20 = — 34° (c = 1.5, chloroform), [o ]d20 = — 43° (c = 1.8, acetic acid). 

Difructose anhydride I was first prepared by Jackson and Goergen.18-70 These authors found a molecular weight of 307 for the anhydride and 574 for the acetate, values which indicate its difructose anhydride composition. Almost simultaneously, Irvine and Stevenson71 prepared the acetate of what they believed to be an anhydrofructose by the action of concentrated nitric acid on inulin acetate. This anhydride was later shown72 to be identical with the difructose anhydride I of Jackson and Goergen. Hexamethyldifructose anhydride I waB hydrolyzed by Haworth and Straight7 and the trimethyl-D-fructose that was formed was identified as 3,4,6-trimethyl-D-fructofuranose by quantitative conversion... 

Protonic activation of o-fmctose, sucrose, or inulin presents serious limitations when looking for pure individual DFA standards due to the reversibility of the dimerization and isomerization processes. Beyond a certain enrichment of the DFA mixtures in kinetic (e.g., 10) or thermodynamic components (e.g., 9), selectivity is rather poor and chromatographic purification is difficult. The use of protecting groups to block the cyclic form of the o-fmctose precursor was first explored by Defaye and coworkers in 1988. Activation of inulin acetate with HF in sulfur dioxide afforded a three-component mixture of the tetra-O-acetyl-p-o-fmctofuranosyl... 

Scheme 7 FIF-catalyzed synthesis of DFAs from inulin acetate...

A procedure that is described by Tollens and Eisner4 is probably suitable for materials less pure than fresh dahlia juices. The juice is expressed in the presence of calcium carbonate, fermented at 25° with baker s yeast, defecated with lead acetate and filtered. After removal of the excess lead with hydrogen sulfide, the filtrate is frozen and thawed to cause the separation of inulin. 

Haworth, Hirst and Percival23 methylated inulin by first acetylating and subsequently converting the acetate to the methyl derivative by means of dimethyl sulfate and sodium hydroxide in acetone solution, a procedure described by Haworth and Streight.24 They hydrolyzed trimethylinulin and quantitatively determined the amounts of trimethyl-D-fructose and tetramethyl-D-fructose formed. There was obtained 3.7%... 

They prepared the acetate by means of acetic anhydride in dry pyridine and purified it by separation from an acetic acid solution to which warm methyl alcohol had been added n ]n2 = — 22.70° (c = 10, acetic acid), m. p. 206-208°. In the presence of barium hydroxide irisin formed an insoluble product whose composition was represented by 6(C6Hi0O6)-Ba(OH)2. In contrast to inulin, these authors found that Takadiastase at pH 5 to 5.5 did not hydrolyze irisin. 

Inulin nitrate is insol in w, eth, ethyl and methyl ale, toluene and carbon tetrachloride, but dissolves in acetone, ether ale mixt, coned H2S04, ethyl acetate etc. It does not crystallize from solutions but forms a film... 

Cirsium chinense Gardn. et Champ. C. japonicum DC Chinese Ji Da Ji (Thistle) (leaf, stem) Alpha-amyrin, beta-amyrin, beta-sitosterol, stigmasterol, taraxsteryl acetate, inulin, labenzyme, pcctolinarin.50 60 Hemostat, diuretic, treat intestinal bleeding caused by ulcers, externally for abscesses and scabies. 

Codonopsis pilosula (Franch.) Nannfeldt C. tangshen Oliv. China Taraxeryl acetate, friedelin, n-butyl allophanate, inulin, sucrose, amino acids, stigmasterol, spinasterol, methyl palmitate, taraxerol.48 For amnesia, anorexia, asthma, cachexia, cancer, impotence, insomnia, palpitations. 

Fuller s earth (hydrated aluminosilicate) Magnesium oxide Charcoal Alumina Magnesium trisilicate Silica gel Calcium hydroxide Magnesium carbonate Calcium phosphate Calcium carbonate Sodium carbonate Talc Inulin Sucrose = starch Petroleum ether, b 40-60°- Petroleum ether, b 60-80°. Carbon tetrachloride. Cyclohexane. Benzene. Ethyl ether. Chloroform. Ethyl acetate. Acetone. Ethanol. Methanol. Pyridine. Acetic acid. 

Succinic acid has a diverse range of applications in the synthesis of specialty chemicals used in agriculture, foods, medicine, textiles, plating, and waste-gas scrubbing (Winstrom, 1978). Currently, succinic acid is produced via the hydrogenation of maleic anhydride to succinic anhydride and hydration to succinic acid (Winstrom, 1978 Zeikus et al., 1995). The thermophylic anaerobic bacteria Clostridium thermosuccinogenes can convert inulin to succinate and acetate as major... 

Inulin can be modified to compounds that display good heavy metal complexing properties similar to ethylene diamine tetra-acetic acid (EDTA) but with better biodegradation properties (Bogaert et al., 1998). Inulin is first oxidized using sodium periodate to the dialdehyde, and then reduced to a polyol using Pt/C and hydrogen. The polyol can then be modified with carbon disulfide to form xanthate or with S03-pyridine to obtain an inulin sulfate. Alternatively, the dialdehyde can be animated with diaminoethane and sodium cyanoborohydride and the product reacted with monochloroacetic acid sodium salt to form carboxymethylamino inulin. Each of these compounds can be used to precipitate heavy metals. 

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