Interleukin measurement

The secondary stmcture elements are then identified, and finally, the three-dimensional protein stmcture is obtained from the measured interproton distances and torsion angle parameters. This procedure requites a minimum of two days of nmr instmment time per sample, because two pulse delays are requited in the 3-D experiment. In addition, approximately 20 hours of computing time, using a supercomputer, is necessary for the calculations. Nevertheless, protein stmcture can be assigned using 3-D nmr and a resolution of 0.2 nanometers is achievable. The largest protein characterized by nmr at this writing contained 43 amino acid units (51). However, attempts ate underway to characterize the stmcture of interleukin 2 [85898-30-2] which has over 150 amino acid units. 

C. Privat, O. Stepien, M. David-Dufilho, A. Brunet, F. Bedioui, P. Marche, J. Devynck, and M.-A. Devynck, Superoxide release from interleukin-1 (3-stimulated human vascular cells in situ electrochemical measurement. Free Radicals Bio. Med. 27, 554-559 (1999). 

KOning A, Muhlbauer RC, Fleisch H (1988) Tumor necrosis factor alpha and interleukin-1 stimulate bone resorption in vivo as measured by urinary [3H]tetracycline excretion from prelabeled mice. J Bone Miner Res 3 621-627... 

Figure 13.1 Microcalorimetry scans displaying Tm values for interleukin-1 receptor (IL-1R type I). The inlay displays the unfolding of IL-1R (I) showing the ACp measured as the baseline difference between the native (N) and denatured (D) states for two independent scans. Thermal unfolding of IL-1R (I) is composed of three cooperative unfolding transitions, labeled 1, 2, and 3.

Interleukin induction. Water extract of freeze-dried grain was active. Interleukin -1 activity was measured by the interleukin-1 dependent growth of a T-helper cell line . 

In five patients who presented in Tasmania during 1 year, all of whom were taking amiodarone 200 mg/day, serum TSH was undetectable and the free thyroxine and triiodothyronine concentrations were raised (46). In one case there was a low titer of TSH receptor antibodies and in another a high titer of antithyroid peroxidase antibodies. In all cases the hyperthyroidism was severe and occurred after at least 2 years of treatment with amiodarone. In one of two patients in whom it was measured the serum concentration of interleukin-6 was raised, as has been previously shown (SEDA-19, 193). In two cases the hyperthyroidism was refractory to treatment with propylthiouracil, lithium, and dexamethasone in these cases thyroidectomy was required. Two patients responded to propylthiouracil, lithium, and dexamethasone, and one responded to carbimazole. 

For the present open observation on sublingual immunotherapy (SLIT), nonspecific inflammatory markers were chosen, which are supposed to be involved in the allergic cascade and which can be measured by common ELISA techniques soluble intercellular adhesion molecule-1 (sICAM-1), sE-selectin, soluble interleukin-2 receptor (sIL-2R), interleukin-12 (IL-12) and specific IgG4. Earlier investigations in this field were mostly performed on a smaller number of patients and the results are sometimes contradicting. 

Taniguchi, M., Nagaoka, K., Kunitake, T., Kayano, T., Yamauchi, H., Nakamura, S., Ikeda, M., Orita, K., and Kurimoto, M. (1997). Characterization of anti-human interleukin-18 (IL-18) /interferon-gamma-inducing factor monoclonal antibodies and their application in the measurement of IL-18 by ELISA. J. Immunol. Methods 206 107. 

---