Integrase protein

Lutzke, R.A.P., Eppens, N.A., Weber, P.A., Houghten, R.A., and Plasterk, R.H.A. Identification of a hexapeptide inhibitor of the human immunodeficiency virus integrase protein... 

Gateway cloning technology is a modification of the recombination system of phage k (Walhout et ah, 2000 Hartley et ah, 2000). The Gateway system utilizes a minimum set of components of the k system for in vitro transfer of DNA, namely the k integrase protein, k excisionase, the E. coli protein... 

Three-dimensional structural information has not yet been obtained for a full-length integrase protein. In its absence, attempts have been made to determine the structure of the smaller domains consisting of the separately expressed N- and C-termini that flank the core whose structure is now known. 

Van Gent DC, Oude Groeneger AAM, Plasterk RHA. Mutational analysis of the integrase protein of human immunodeficiency virus type 2. Proc Natl Acad Sci USA 1992 89 9598-9602. 

Vink C, Oude Groeneger AAM, Plasterk RHA. Identification of the catalytic and DNA-binding region of the human immunodeficiency virus type 1 integrase protein. Nucl Acids Res 1993 21 1419-1425. 

Puras Lutzke RA, Vink C, Plasterk RHA. Characterization of the minimal DNA-binding domain of the HIV integrase protein. Nucl Acids Res 1994 22 4125-4131. 

Bushman FD, Wang B. Rous sarcoma virus integrase protein Mapping functions for catalysis and substrate binding. J Virol 1994 68 2215-2223. 

Engelman A, Hickman AB, Craigie R. The core and carboxyl-terminal domains of the integrase protein of human immunodeficiency virus type 1 each contribute to nonspecific DNA binding. J Virol 1994 68 5911-5917. 

Famet CM, Wang B, Lipford JR, Bushman FD. Differential inhibition of HIV-1 preintegration complexes and purified integrase protein by small molecules. Proc Natl Acad Sci USA 1996 93 9742-9747. 

Ubeda C, Tormo MA, Cucarella C, Trotonda P, Foster TJ, Lasa I, Penades JR Sip, an integrase protein with excision, circularization and integration activities, defines a new family of mobile Staphylococcus aureus pathogenicity islands. Mol Microbiol 2003 49 193-210. 

From a mechanistic aspect, the RIPs GAP 31 (from Gdonium multiflorum) [77], luffin (from Luffa cylindrica) and saporin (from Saponaria officinalis) [78] were reported to inhibit the HIV-1 integrase protein. 

These two sites contain identical 15-bp core sequences within which the recombination occurs. In a manner similar to that of the recA protein a homologous region is located by the complex of the Int (integrase) protein and IHF. Several molecules of Int protein bind and, together with the IHF protein, hold the phage DNA in a nucleosomelike structure (an intasome) in which the recombination occurs Strand cleavage... 

In addition to the G-quadruplex other motifs that have been solved include the i-motif, a pH-dependent poly dA helix which forms the basis of a molecular switch, the HIV-1 TAR DNA hairpin, and the B-Z transition in a GC-rich DNA sequence. There are also many protein/ peptide structures that have been solved that have DNA as part of the recognition domain, including a zinc sensor, MetJ repressor, lac repressor, Lambda integrase protein, protection of telomers (Potl)... 

---