Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Gateway cloning

Gateway cloning technology is a modification of the recombination system of phage k (Walhout et ah, 2000 Hartley et ah, 2000). The Gateway system utilizes a minimum set of components of the k system for in vitro transfer of DNA, namely the k integrase protein, k excisionase, the E. coli protein... [Pg.24]

Fig. 2.8 Three-fragment Multisite Gateway cloning. Creation of entry vectors and three-fragment Multisite assembly reaction. Fig. 2.8 Three-fragment Multisite Gateway cloning. Creation of entry vectors and three-fragment Multisite assembly reaction.
The full power of this system is realized in the Multisite Gateway system, where the same mechanism which is used to transfer single DNA elements from vector to vector is employed to assemble multiple fragments. The future of Gateway cloning will include HTP, controllable assembly of multi-element plasmids, multi-domain proteins, and entire enzymatic pathways that could be expressed in a desired cellu-... [Pg.619]

The ability efficiently to manipulate DNA elements afforded by the Gateway cloning system has opened (see also Part III, Chapters 1 and 7) - and wiU continue to open - many doors to novel experimental protocols that wiU be invaluable in basic research finked with the development of modem biopharmaceuticals. [Pg.619]

WaUiout, A. J., Temple, G. E, Brasch, M. A., Hartley, J. L., Lorson, M. A., van den Heuvel, S. and Vidal, M. (2000). GATEWAY recombinational cloning application to the cloning of large numbers of open reading frames or ORFeomes. Method Enzymol. 328, 575-92. [Pg.43]

Systematic Cloning of an ORFeome Using the Gateway System... [Pg.11]

Key words ORFeome, The Gateway system, Cloning, High-throughput, Schizosaccharomycespombe... [Pg.11]

See other pages where Gateway cloning is mentioned: [Pg.241]    [Pg.6]    [Pg.7]    [Pg.7]    [Pg.8]    [Pg.10]    [Pg.614]    [Pg.614]    [Pg.614]    [Pg.616]    [Pg.619]    [Pg.1857]    [Pg.241]    [Pg.6]    [Pg.7]    [Pg.7]    [Pg.8]    [Pg.10]    [Pg.614]    [Pg.614]    [Pg.614]    [Pg.616]    [Pg.619]    [Pg.1857]    [Pg.39]    [Pg.4]    [Pg.24]    [Pg.24]    [Pg.9]    [Pg.9]    [Pg.11]    [Pg.12]    [Pg.13]    [Pg.13]    [Pg.14]    [Pg.15]    [Pg.24]    [Pg.124]    [Pg.54]    [Pg.167]    [Pg.387]    [Pg.705]    [Pg.360]    [Pg.150]   
See also in sourсe #XX -- [ Pg.72 , Pg.73 ]



SEARCH



Applications Enabled by Gateway Cloning

Cloning system Gateway

Learning from Viruses High-throughput Cloning using the Gateway System to Transfer Genes without Restriction Enzymes

© 2024 chempedia.info