Inhibitory activity Bacillus subtilis

Anti-amoebic activity. Ethanol (80%) extract of the dried rhizome was inactive on Entamoeba histolytica, minimum inhibitory concentration (MIC) greater than 1 mg/ mL The extract, administered intragas-trically to male hamsters at a dose of 800 mg/kg, was active vs experimentally induced hepatic amebiasis . A dose of 250 mg/kg, administered intragastrically to rats on days 1-5, produced weak activity and a dose of 500 mg/kg was active " ". Anti-atherosclerotic activity. Ethanol (50%) extract of the dried rhizome, administered intragastrically to male rabbits at a dose of 500 mg/kg, reduced atherogenic index from 4.7 to 1.2 on the aorta . Antibacterial activity. Decoction of the dried entire plant, on agar plate, was inactive on Proteus vulgaris, Staphylococcus aureus, and Staphylococcus epidermidis MIC 125 mg/mL. Bacillus subtilis, Bordetella... 

Table 7. Inhibitory activity against Bacillus subtilis H17 and tec-assay (disk diffusion method) of isoprenoid-substituted phenols (75 pg/disk), their cytotoxic activities against HSC-2 and MT-4 cells, and other biological activities...

Further to these results, the microbicidal activities of FIBCIDE-KF and KATHON WT were evaluated. The minimum inhibitory concentrations (MIC) for Pseudomonas aeruginosa, Pseudomonas putida, Bacillus subtilis and Microbacterium sp. are identical for both compounds, so the microbicidal effect has not diminished (Figure 10). It appears that the biocidal effects are similar because the Cl-MIT in the inclusion complex is released into water, as shown in Figure 6. 

Organotin complexes of di(2-pyridyl) ketone 2-thenoylhydrazone, 1 and 2, with 6- and 5-coordinated tin atom are less active than the parent organotins67,68. Compound 2 showed good results in tests against Bacillus subtilis, Staphylococcus aureus and also toward several Bacilli with minimum inhibitory concentration ranging from 1.5 to 3 pgml-167. At the same time this complex is devoid of DNA-damaging activity in the Bacillus subtilis rec-... 

Besides ACE-inhibitory and antioxidative activities, other biological properties have been reported for protein hydrolysates and their associated bioactive peptides. For example, earlier studies demonstrated a hypolipidemic effect of fish protein (Bergeron and Jacques, 1989 Zhang and Beynen, 1993). A recent study by Tanaka et al. (2006) demonstrated production of protein hydrolysates from oyster prepared with aloase, an endoprotease from Bacillus subtilis, and... 

Finally, bacitracins are peptide antibiotics produced by Bacillus subtilis and B. licheniformis. Over 20 components are contained in the bacitracin complex medium, among which the major active components are bacitracin A and F (Fig. 3). They exhibit an inhibitory activity against Gram-positive bacteria and are among the most commonly used antibiotics as animal feed additives. 

Huang, M.-J. and Bodor, N. (1994). Quantitative Structure-Inhibitory Activity Relationships of Substituted Phenols on Bacillus Subtilis Spore Germination. Int.J.Quant.Chem., 181-185. 

The inhibitory effect on Bacillus subtilis is being reversed by high concentration of potassium salts (e.g. 0.2 M KCl). The reversion is specific for potassium salts. Addition of boromycin is followed by a discharge of potassium ions from the cells. The Na -activated ATPase of the cytoplasmic membrane is not influenced by boromycin. The degradation of boromycin through alkaline and acid hydrolysis leads to a loss of antibiotic activity, due to the boric acid splitting off from the molecule. 

Xanthocillin-X monomethylether (XME), dimethylether (XDE), and itiethoxy xanthocillin-X dimethylether (XTE) have been isolated from Dendotomyces albus and from two strains of Asp. chevalieri by Suzuki and co-workers (258). All these compounds decompose near 183°C and are effective against Newcastle disease and inhibit the growth of Bacillus subtilis. XME specifically inhibits the conversion of arachidonic acid to prostaglandin H2 (259) and shows an inhibitory activity against platelet aggregation. 

Both the antibacterial and haemolytic activities increased wheji the alkyl chain length was >C6. The activity of each polymer at two different MW (Mn, th = 3 and 10 kDa) was determined against Escherichia coli. Bacillus subtilis and human RBC (Table 9.1). Haemolytic activity was assessed as HCsolthe concentration of peptide required for 50% haemolysis), while the reported minimum inhibitory concentration (MIC) caused greater than 90% inhibition and was typically resulted in >99% inhibition. Increasing the alkyl substituent chain length caused an increase in activity, which indicates that hydrophobicity plays a major role in the antibacterial activity of the polymers. 

Agaricone (549), xanthodermin (551) and the diazo compounds (547) and (548) are antibiotically active against Bacillus subtilis and B. brevis 367). Especially active against both bacteria and fungi are the two diazo compounds the minimum inhibitory concentrations of which are comparable with those of established antibiotics (795, 367). 

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