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Incorporation of protein

The mechanistic properties are clearly improved by the incorporation of proteins. Insects and crustaceans have a shell made of chitin, a chain molecule (polymer) based on chemically modified (acetylamino-) glucose. [Pg.90]

Leonor, I.B., Azevedo, H.S., Alves, C.M. and Reis, R.L. (2003) Effects of the incorporation of proteins and active enzymes on biomimetic caldum-phosphate coatings. Key Engineering Materials, 240-242, 97—100. [Pg.366]

Le Gaherec F, Bron P, Verbavatz JM, Garret A, Morel G, et al. 1996. Incorporation of proteins into (Xenopus) oocytes by proteoliposome microinjection functional characterization of a novel aquaporin. J Gell Sci 109 (Pt 6) 1285. [Pg.340]

Osterberg, F., Morris, G.M., Sanner, M.F., Olson, A.J. and Goodsell, D.S. (2002) Automated docking to multiple target structures incorporation of protein mobility and structural water heterogeneity inAutoDock. Proteins, 46, 34—40. [Pg.80]

The compartmentation of cubic phases is geometrically not so well defined as in the case of micelles or vesicles. However, several years ago the very interesting observation was made that cubic phases can incorporate proteins up to 50% of their weight (Ericsson et al, 1983). Usually cubic phases also remain transparent after incorporation of proteins, and in fact it has been possible to carry out circular dichroic investigations of enzymes in such systems, (Larsson, 1989 Portmann et al, 1991 Landau and Luisi, 1993), as shown in Figure 9.19, and even to follow spectroscopically the course of enzymatic reactions (Portmann et al, 1991). [Pg.198]

Methods were described for the incorporation of proteins in the form of noncovalent complexes with polycationic reagents, into sustained release systems where the polycation stabilizes the protein against inactivation while it resides in the delivery device, and retards release of the protein from the delivery device [469,470]. A variety of polycations have been used, including simple polyamino acids such as polylysine or polyarginine, protamine and chitosan. The end result was the release of the active agent with retention of biological activity, with a high cumulative field and over a sustained period of time. [Pg.39]

Lipid micellization Vesicles for encapsulation, controlled release, functional incorporation of proteins and mimicking of biological membranes Singh et al., 1995 Taylor et al., 2005 Sagalowicz et al., 2006 Mozafari et al., 2006... [Pg.12]

As presented, data are available on assessment of water absorption in simple systems and on the incorporation of protein additives into some food systems. Functional properties in... [Pg.196]

One of the major goals of these many investigations of lipids is, of course, a better understanding of the in - vivo behavior of membranes. Beyond studies of binary lipid mixtures, as mentioned above, a further step which is necessary is the incorporation of proteins into the layers. In many respects, this increase in the complexity of the bilayer systems resembles that encountered in the use of synthetic surfactants in "real - world" situations, where blends, rather than single, surfactants are used. Surfactant blends in aqueous solutions are often further modified in use by the solubilization of oily organic compounds, as in the cases of detergency or cosmetic formulation. [Pg.9]

The encapsulation of bioactive agents, such as proteins or peptides, presents a special problem due to the delicacy of their structural conformation. Their biological activity may be irreversibly disturbed even by small changes of pH, temperature, or ionic concentration [192,200]. Spray-drying and double emulsion solvent evaporation techniques (w/o/w) have been suggested for the incorporation of proteins with retention of their bioactivity [99,192,202,198]. [Pg.101]

Lill MA (2011) Efficient incorporation of protein flexibility and dynamics into molecular docking simulations. Biochemistry 50(28) 6157-6169... [Pg.11]

Acetylcholineesterase Biosensors were fabricated from filter-supported solventless bilayer lipid membrane (BLM) and used for the analysis of the substrates of hydrolytic enzymes in a flowthrough system. The codeposition of lipid (dipalmitoyl-phosphatidic acid) and protein solutions to form a BLM on a microporous glass fiber or polycarbonate ultra-filtration membrane disc was described. Enzyme was immobilized on the membrane by incorporation of protein solution into the lipid matrix at the air-electrolyte interface before BLM formation. [Pg.51]

Sturesson, C., and Carlfors, J. (2000), Incorporation of protein in PLG-microspheres with retention of bioactivity, J. Controlled Release, 61,171-178. [Pg.436]

The rheological behavior of systems containing starch, soybean protein, soybean oil, and water (5 10 10 40) has been studied.1038 The viscosity of the system depends on the origin of the starch. For example, the viscosity of a potato-starch gel markedly decreases upon the addition of protein. In contrast, a cornstarch gel is much more stable and does not show a decrease in viscosity after the addition of protein. The incorporation of proteins into a lipid-starch system shows that proteins are distributed among both components of the system.885... [Pg.411]

Incorporation of Proteins into Polymerized Cubic Phases. [Pg.217]

At the present time, 2,3-methanoamino acid analogs can only be viewed as reagents in the context of syntheses of peptidomimetics. Consequently, this entry describes only that chemistry related to incorporation of protein amino acid methanologs into peptide sequences. [Pg.200]

In all the biomimetic membranes previously described and allowing the incorporation of proteins, the protein orientation in the membrane is purely casual. At most, if one of the two extremembrane domains of the protein is much bulkier than the other, incorporation in a tBLM occurs preferentially with the bulkier domain turned toward the aqueous phase, in view of the hmited spaciousness of the hydrophihc moiety of the tBLM. Moreover, the packing density of the reconstituted proteins in the hpid bilayer is not well controlled. The need for a well-defined protein orientation with respect to the electrode surface is particularly felt with redox membrane proteins, in which the electrons involved in a chain of redox couples are conveyed across the membrane in a weh-defined direction. [Pg.220]

PLGA is the most widely used polymer for encapsulating proteins for pulmonary delivery. The most commonly used method for preparing protein-encapsulated PLGA microspheres is the solvent evaporation technique based on the formation of a double emulsion (w/o/w). Incorporation of protein into the microspheres could be done by two methods [15, 16]. [Pg.144]

The ability of polyelectrolytes to remove oppositely charged proteins from solutions has been exploited through the incorporation of protein-polymer precipitation steps into a variety of protein purification procedures, wherein precipitated proteins are recovered from the insoluble complex aggregate via redissolution by pH or ionic strength adjustment (14-16). Furthermore, preferential complexation of polyelectrolytes with specific proteins has been substantiated (13). Although there are reports of the optimization of bulk complexation yield through the adjustment of solution parameters such as pH and ionic strength (17), very little has been accomplished in the optimization of the selectivity of complex formation. [Pg.159]

Watson MS, Whitaker MJ, Howdle SM, Shakesheff KM. Incorporation of proteins into polymer materials by a novel supercritical fluid processing method. Adva Mate 2002 14 1802-1804. [Pg.489]

Next, the use of polyelectrolyte multilayers, prepared by the layer-by-layer deposition protocol, as well as the use of polymer cushions prepared by plasma-polymerization is introduced. Evidence for the proper structural and functional characteristics of the corresponding tethered bilayers is derived from neutron reflectivity and from IR data, and by the observation of the functional incorporation of proteins. [Pg.88]

Pioneering work in the incorporation of functional proteins into polymer bilayers was performed by Meier et al., who integrated membrane proteins into black block copolymer membranes [250], This work proved that proteins could be incorporated into hyperthick triblock copolymer membranes while maintaining their functionality as measured by membrane conductance. Incorporation of proteins in black block copolymer films has been expanded for applications in sensors [251] and protein driven energy transduction [252] across polymeric biomembranes. [Pg.155]

Another computational search was performed using a pharmacophore model based on the crystal structure of MDM2 with incorporation of protein flexibility assessed using molecular dynamics simulation [47]. Pharmacophore searching was performed on a database of 35,000 synthetic compounds, followed by evaluation of 24 hits in a fluorescence-polarization MDM2 binding assay that led to the discovery of five non-peptidic, small-molecule MDM2 inhibitors with new scaffolds. [Pg.63]

It is well known that ferrous iron is absorbed from the intestinal tract of animals much more rapidly than ferric iron and that ascorbic acid can reduce ferric to ferrous iron. Additional proofs of the facilitation of ferric iron absorption by ascorbic acid have been reported (Cll, B29, W5). The same action in a cell-free system was demonstrated with the stimulation by ascorbic add of the incorporation of protein-bound ferric iron into protoporphyrin (L20). This enzyme reaction is known to require a reducing agent, and as these workers demonstrated, glutadiione functions equally as well as ascorbic add in this regard. [Pg.162]


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See also in sourсe #XX -- [ Pg.217 ]




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