In Vitro Cell Growth

In order to determine whether a biomaterial s surface is suitable for a later cell seeding, proliferation studies with HUVEC are carried out over several days. 

The evaluation is done by measurement of the cell density after the first, fourth and seventh cultivation day at the samples s surface and by comparison of these values with those obtained in the positive control Thermanox [92]. 

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Replacement of the A -methyl group of MTX by larger, more lipophilic substituents has been studied for its effects on DHFR binding, membrane transport, in vitro cell growth inhibition, and in vivo antitumour activity [14, 63-65]. 

Kopf-Maier P, Wagner W and Kopf H (1981) In vitro cell growth inhibition by metallocene dichlorides. Cancer Chemother Pharmacol 5 237—241. 

Badylak SF, Record R,Lindberg K,Hodde J, Park K. Small intestinal submucosa a substrate for in-vitro cell growth. J. Biomater. ScL Polym. Ed. 9 863-878,1998. 

Cell culture approaches to investigate in vitro cell growth behavior. 

Au nanoparticles in PU + cell seeding Spin casting CD31 expression, tissue response In vitro rabbit in vivo Endothelial cells rabbit tissue Best in vitro cell growth on PU—Au, in vivo more endothehahzation [84]... 

McCormick and coworkers polymerized NIPAm at room temperature via aqueous RAFT process [59]. Extending the study to prepare poly(A,A-dimethyl acrylamide(DMA)- -NIPAm) diblock and poly(DMA-fc-NIPAm- -DMA) triblock copolymers, the group demonstrated that critical micelle temperature (CMT) was lower for diblock than for triblock copolymers, and decreased as the PNIPAm block length was increased [60]. The phase transition temperature of this polymer system may be adjusted from 10 °C to 36 °C by incorporating an additional pH-responsive block [61]. Thermoreversible hydrogels from RAFT-synthesized poly(NIPAm- -DMA- -NIPAm) triblock copolymers were also investigated as potential in vitro cell growth scaffolds [62]. 

Badylak, SF, Record, R, Lindberg, K, Hodde, J, and Park, K. 1998. Small intestinal submucosa A substrate for in vitro cell growth. /Biomater Sci Polym Ed 9(8) 863-878. 

Hatzinger, P.B. and Stevens, J.L. (1989). Rat kidney proximal tubule cells in defined medium the roles of cholera toxin, extracellular calcium and serum in cell growth and expression of y-glutamyltransferase. In Vitro Cell. Dev. Biol. 25(2) 205-212. 

Several black tea derived polyphenols, including theaflavin-3,3 -digallate, can inhibit in vitro the growth of human esophageal squamous carcinoma cells at concentrations near 20 and theaflavin monogallates have in vitro inhibited growth of human colon cancer... 

Wilson, D. A., Weigel, R. C., Wheeler, R. M., Sager, J. C. (1993). Light spectral quality effects on the growth of potato Solanum tuberosum L.) nodal cuttings in vitro. In Vitro Cell Develop Biol., 29, 5-8. 

Sundqvist, K., et al. 1991. Growth regulation of serum-free cultures of epithelial cells from normal human buccal mucosa. In Vitro Cell Dev Biol 27A 562. 

Ikonomou L, Bastin G, Schneider YJ, Agathos SN (2001), Design of an efficient medium for insect cell growth and recombinant protein production, In Vitro Cell. Dev. Biol. Anim. 37 549-559. 

Cohen, S. (1987). Epidermal growth factor. In Vitro Cell Dev. Biol. 23, 239-246. 

The feet that mutant Ras genes have been associated with a wide variety of human tumors suggests that they are responsible for the uncontrolled growth of tumor cells. Studies involving in vitro cell culture and transgenic animals have demonstrated the ability of... 

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