Immobilization of glucose oxidase

Fig. 4.29 Immobilization of glucose oxidase in mesocellular carbon foam for a sensitive and fast glucose biosensor. Adapted from [226], D. Lee et a ., Adv. Mater. 2005, 77, 2828.

Z. Zhang, H. Liu, and J. Deng, A glucose biosensor based on immobilization of glucose oxidase in electropolymerized o-aminophenol film on platinized glassy carbon electrode. Anal. Chem. 68, 1632-1638 (1996). 

J. Yu, S. Liu, and H.X. Ju, Glucose sensor for flow injection analysis of serum glucose based on immobilization of glucose oxidase in titania sol-gel membrane. Biosens. Bioelectron. 19, 401—409 (2003). 

Glucose Immobilization of glucose oxidase, peroxyoxalate-H202 CL 0.05 pM 42... 

Antigen-Antibody Immobilization of Glucose Oxidase. Kinetic Analysis... 

The following experiment illustrates the immobilization of glucose oxidase in agarose (Gutcho, 1974). 

Garwood, G. A., Mortland, M. M., and Pinnavaia, T. J. (1983). Immobilization of glucose oxidase on montmorillonite clay Hydrophobic and ionic modes of binding. J. Mol. Catal. 22,153-163. 

Pishko MV, Michael AC, Heller A. Amperometric glucose microelectrodes prepared through immobilization of glucose-oxidase in redox hydrogels. Analytical Chemistry 1991, 63, 2268-2272. 

Miscoria S, Barrera G, Rivas G. Glucose biosensors based on immobilization of glucose oxidase and polytyramine on rodhinized glassy carbon and screen printed electrodes. Sensors and Actuators B 2006, 115, 205-211. 

This study on the immobilization of glucose oxidase and the characterization of its activity has demonstrated that a bioactive interface material may be prepared from derivatized plasma polymerized films. UV/Visible spectrophotometric analysis indicated that washed GOx-PPNVP/PEUU (2.4 cm2) had activity approximately equivalent to that of 13.4 nM GOx in 50 mM sodium acetate with a specific activity of 32.0 U/mg at pH 5.1 and room temperature. A sandwich-type thin-layer electrochemical cell was also used to qualitatively demonstrate the activity of 13.4 nM glucose oxidase under the same conditions. A quantitatively low specific activity value of 4.34 U/mg was obtained for the same enzyme solution by monitoring the hydrogen peroxide oxidation current using cyclic voltammetry. Incorporation of GOx-PPNVP/PEUU into the thin-layer allowed for the detection of immobilized enzyme activity in 0.2 M sodium phosphate (pH 5.2) at room temperature. 

Electron-Mediated Biosensor. Electropolymerization of 1,3-diaminobenzene (1,3-DAB), followed by adsorption of l,r-dimethylferrocene (1,1 -DMF), and immobilization of glucose oxidase, results in an easily and quickly (<2 h) constructed glucose biosensor with excellent linearity and stability (>3 months). Figure 5 shows a proposed schematic of the sensing layer consisting of film/mediator/enzyme. The ferrocene is depicted as circles... 

Immobilization of glucose oxidase via carbodiimide chemistry onto a poly(acrylic acid) grafted porous cellulose substrate was performed by Ito, et al. (14). They showed that this system s permeability to insulin in response to a 3600 mg% glucose challenge was 1.7 times greater than the permeability prior to the addition of glucose. 

Xue, M. H., Xu, Q., Zhou, M. and Zhu, J. J. (2006), In situ immobilization of glucose oxidase in chitosan-gold nanoparticle hybrid film on prussian blue modified electrode for high-sensitivity glucose detection. Electrochem. Commun., 8(9) 1468-1474. 

The principal application of lectins in bioanalytical systems involves the reversible immobilization of glucose oxidase, invertase, and peroxidase on Con A-Sepharose. Such lectin-based affinity media have also been utilized for immobilization of glycoenzymes. Woodward (18) shows that cellobiase is not desorbed by its substrate cellobiose and product glucose from the support matrix. 

Our measures to overcome these problems include the preparation of aldehyde-functionalized silanes as a new class of spacer molecules for silica surfaces, the application of a series of such compounds with up to three ethoxy groups and different chain length of the anchor group to the surface of controlled pore glass (CPG) as a model substrate, the immobilization of glucose oxidase (GOD) and systematic activity studies over a period of four weeks. 

Immobilization of Glucose Oxidase. The enzyme glucose oxidase was incorporated into the aqueous phase of a cubic phase similar to that polymerized in the previous section, and this aqueous phase made polymerizable by the addition of monomeric acrylamide. Except for a slight yellowish color from the strongly-colored glucose oxidase, the result was an optically clear polymerized materiaL TTie concentration of... 

A highly sensitive glucose biosensor based on immobilization of glucose oxidase ( GOx) onto tetragonal pyramid-shaped (TPSP) ZnO nanostructure is prepare [164], TPSP- ZnO nanostructure exhibits favorable biocompability for facilitating the electron transfer between... 

Figure 28. CVs of GOx /NiOx modified GC electrode at various scan rate in pH 7 PBS, from inner to outer, 10, 20, 30, 40, 50, 60, 70, 80, 90 and lOOmVs 1. Reprinted from Biosensors and Bioelectronics, 22, ASalimi, E. Sharifi, A. NoorBakhash, S. Soltanian, Immobilization of glucose oxidase on electrodeposited nickel oxide nanoparticles Direct electron transfer and electrocatalytic activity,3148,Copy eight (2007), with permission from Elsevier.

S. Soltanian, Immobilization of glucose oxidase on electrodeposited nickel oxide nanoparticles Direct electron transfer and electrocatalytic activity,3151, Copyeight (2007), with permission from Elsevier. 

Foulds, N.C. and Lowe, C.R. (1988) Immobilization of glucose oxidase in ferrocene-modified pyrrole polymers. Analytical Chemistry, 60, 2473-2478. 

Li ZF, Kang ET, Neoh KG, Tan KL (1998) Covalent immobilization of glucose oxidase on the surface of polyaniline films graft copolymerized with acrylic acid. Biomat 19 45-53... 

Immobilization of glucose oxidase on planar electrode arrays in photopolymerized poly-2-hydroxy-ethylmethacrylate membranes Glucose [23]... 

Iwakura, C. Kajiya, Y. Yoneyama, H. Simultaneous immobilization of glucose oxidase and a mediator in conducting polymer films. J. Chem. Soc., Chem. Commun. 1988, 1019-1020. 

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