Hydrolyzing enzyme activity

DeFrank 11, WT Beaudry, T-C Cheng, SP Harvey, AN Stroup, LL Szafraniec (1993) Screening of halophiliuc bacteria and Alteromonas species for organophosphorus hydrolyzing enzyme activity. Chem-Biol Interact 87 141-148. 

Oi, Y., Kawada, T., Wantanabe, T., Iwai, K.J. (1992). Induction of capsaicin-hydrolyzing enzyme activity in rat liver by continuous oral administration of capsaicin. J. Agric. Food Chem. 40 467-70. 

Oi Y, Kawada T, Watanabe T et al. (1992). Induction of capsaicin-hydrolyzing enzyme activity in rat liver... 

Ray R, Boucher LJ, Broomfield CA, Lenz DE. Specific soman-hydrolyzing enzyme activity in a clonal neuronal cell culture. Biochim Biophys Acta. 1988 967 373-381. 

Kashiwagi M, Fuhshuku K-1, Sugai T (2004) Control of the nitrile-hydrolyzing enzyme activity in Rhodococcus rhodochrous IFO 15564 preferential action of nitrile hydratase and amidase depending on the reaction condition factors and its application to the one-pot preparation of amides from aldehydes. J Mol Catal B Enzym 29(l-6) 249-258... 

Fungal cutinase catalyzes hydrolysis of model substrates and in particular p-nitrophenyl esters of short chain fatty acids, providing a convenient spectro-photometric assay for this enzyme activity [101,102,116]. Hydrolysis of model esters by this cutinase showed the high degree of preference of this enzyme for primary alcohol ester hydrolysis. Wax esters and methyl esters of fatty acids were hydrolyzed at low rates. Alkane-2-ol esters were hydrolyzed much more slowly than wax esters and esters of mid-chain secondary alcohols were not... 

Pigman,22 in an investigation of the inulin-hydrolyzing enzymes in commercial enzyme preparations, has noted the same pH for optimum activity. He concludes that the inulases and invertases are different enzymes or that they represent a class of enzymes in which the individual members vary according to source. 

Although the inhibition-based biosensors are sensitive, they are poor in selectivity and are rather slow and tedious since the analysis involves multiple steps of reaction such as measuring initial enzyme activity, incubation with inhibitor, measurement of residual activity, and regeneration and washing. Biosensors based on direct pesticide hydrolysis are more straightforward. The OPH hydrolyzes ester in a number of organophospho-rus pesticides (OPPs) and insecticides (e.g. paraoxon, parathion, coumaphos, diazinon) and chemical warfare agents (e.g. sarin) [53], For example, OP parathion hydrolyzes by the OPH to form p-nitrophenol, which can be measured by anodic oxidation. Rainina... 

I. H+-ATPase, H+-PPase, and UDPase. Enzyme activities of vanadate- or nitrate-sensitive H+-ATPase, H+-PPase, and UDPase are measured by the quantitation of released inorganic phosphate from the substrate by enzymatic hydrolyzation. When H+-ATPase activities are assayed, at least three substrate mixtures should be prepared (1) a substrate mixture containing nitrate, (2) a substrate mixture containing vanadate, and... 

Emil Fischer developed a strong interest in the structural requirements for enzyme activity as the result of effects of changes in the structures of the a-and -methyl glucosides on their properties as substrates for the enzymes invertin and emulsin, which, as we have seen, he had shown to be a- and yS-glucosidases, respectively. As already mentioned, he was fascinated in 1895 by the fact that emulsin had no effect on either the a- or /2-methyl xylosides (33). In a 1912 publication with Karl Zach (34), he reported that -methyl 6-deoxyglucoside was hydrolyzed by emulsin and wrote ... 

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