Hybridization Arrays

Freeman WM et al. Fundamentals of DNA hybridization arrays for gene expression analysis. BioTechniques 2000 29 1042-1055. 

Ylstra B, Van den IJssel P, Carvalho B, et al. BAC to the future or oligonucleotides a perspective for micro array comparative genomic hybridization (array CGH). Nucleic Acids Res. 2006 34 445 150. 

E. P. Diamandis and T. K. Christopoulos, Europium chelate labels in time-resolved fluorescence immunoassays and DNA hybridization arrays, Anal. Chem. 62, 1149A-1157A(1990). 

Liu, Y. and Rauch, C.B., DNA probe attachment on plastic surfaces and microfluidic hybridization array channel devices with sample oscillation. Anal. Biochem., 317, 76-84, 2003. 

Edgar R, Domrachev M, Lash AE (2002) Gene expression omnibus NCBI gene expression and hybridization array data repository. Nucleic Acids Res 30 207-210... 

Another unique approach to microarray technology uses fiber optic bundles to support an array of microbeads that are coated with DNA capture sequences. Each bead is individually associated with a single fiber in the bundle, and hybridization targets labeled with fluorescent dyes are hybridized to the beads. The one-to-one concordance of bead to fiber in the bundle permits every bead in the hybridization array to be addressed and reported individually/94,95)... 

A method to form indium bumps for hybrid arrays using dry etching of an indium layer is also disclosed. 

The evolution of imager structures using Mercury Cadmium Telluride (HgCdTe) is described based on published patents and patent applications. The book is divided into two parts. The first describes monolithic arrays, with the detector elements and read-out means integrated in the same semiconductor body. The second part describes hybrid arrays, with the detector elements and the read-out means formed in separate semiconductor bodies. 

The book is separated into a first part with monolithic arrays and a second part with hybrid arrays. Each part comprises five chapters. The documents of each chapter are placed in a chronological order with the documents with earliest priority placed first. The documents are separated by a star Sometimes a document has been placed at a position which is earlier than that which corresponds to its priority date. This has been done when the document shares basic features with a document already presented in the chapter. The document then follows directly after the earlier document and the documents are separated by two stars... 

An alternative approach is to form an hybrid array [20], The detector array is then formed in a mercury cadmium telluride substrate and the readout circuits are formed in a silicon substrate. The hybrid arrays are presented in part two. Detector arrays having detector elements which are provided with individual read-out leads formed in or on a non-active supporting substrate are presented in chapter 2.1. The detector elements may however be directly connected to a read-out chip which is bonded to the detector chip. A flip-chip bonding technique using indium bumps may be used as shown in figure 3 [21]. 

The scientific basis of hybridization arraying as a technique for the determination of gene expression levels is shown in Figures 15.4 and 15.5. The two currently available systems use either immobilized oligonucleotides or immobilized cDNAs as mRNA hybridization targets, but both require preparation and fluorescent-labeling of the mRNAs prior to quantitation. A full description of these hybridization arraying approaches has been published and is also available on the Web (see Table 15.1). 

Hybrid array Ordered mesoporous mixed metal oxide... 

Microdevices and microtechnological developments have had their greatest impact in the field of genomics, and thousands of papers have been published describing their appheation in this arena. In essence the involvement covers several main areas, including techniques for DNA and ampMcation, hybridization arrays, sample preparation, and detection and quantitation of nucleic acids and small polynucleotides (see Chapters 36 and 37). 

Hybridization arrays are now widely used in genomic research (see Chapter 37). Primarily these arrays have been developed to meet the growing demands of pharmaceutical research. However, there are few academic centers today that do not have core facilities to prepare low- and high-density arrays to meet the requirements of academic research studying gene expression. 

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