Core facilities

Core Facilities Commercial Labs Diagnostic Labs... 

This work was supported in part by the Australian Research Grants Council and the Government of Queensland. The authors thank all Alchemia core facilities that participated in these study, especially Mr. Hoan The Vu for his support in analytical tasks and the entire VAST team for their support of this research. 

Although benchtop cytometers are less expensive than state-of-the-art instrumentation, they are still expensive. Therefore core facilities with shared instrumentation still provide for much of the current flow cytometric analysis. These shared facilities reflect a need by many for flow cytometric instrumentation, but also recognition of its high cost, its requirement for skilled maintenance and operation, and the fact that many users from many departments may each require less than full-time access. Such centralized facilities may have more than one cytometer. The trend now is to have one or more sophisticated instruments for specialized procedures accompanied by several bench-... 

SOURCE Photograph courtesy of Peter R. Hoyt, Oklahoma State University Microarray Core Facility, and Mitchel J. Doktycz, Oak Ridge National Laboratory Life Sciences Division. 

Marjam Behar, National Institutes of Health I joined the faculty of the University of Pennsylvania School of Medicine, Department of Anesthesiology, in December 1962 to work with a group of physicians who were doing studies of cerebral blood flow, and they needed a chemist to do their metabolic studies. I didn t have a tenure-track position. As a matter of fact I was not in the faculty track, but as we advanced in the studies (I was there for 17 years), they made me director of the Core Facility for Analytical Chemistry. I had 12 technicians that I supervised and taught. I also taught residents, faculty members, and medical students who needed to learn bioanalytical techniques to pursue their research. 

I was the chemist in the department, we had a center grant, and I was part of the investigators in the center grant as the director of the Core Facility for Analytical Chemistry. But I was not in the tenure track, and it is difficult for a woman in one of these universities to get in the tenure track. If you are a chemist in a department of a medical school it is even more difficult. 

Verifying the identity of cell lines chosen for use should also become a routine task in cell culture laboratories. The number of documented cases of misidentification and cross-contamination of cell lines is growing (Chatterjee 2007). The National Institutes of Health (NIH) issued a notice on November 28, 2007 (NOT-OD-08-017) recognizing the importance of this issue and suggesting that peer reviewers of grant applications and manuscripts submitted for publication should assure that scientists employ available authentication procedures. As the pressure to authenticate cell lines continues, we can expect core facilities and contract research organizations to expand their offerings of such services. 

Mass spectra were obtained with the support of the Mass Spectrometry and Proteomics core facility at the University of Nebraska Medical Center. This work was supported by US Army Medical Research and Material Command W81XWH-07-2-0034 (to OL), NIH CounterACT UOl NS058056-02 (to OL), NIH Eppley Cancer Center grant P30CA36727, and DGA grant 03co010-05/PEA01 08 7 (to P.M.). 

Hybridization arrays are now widely used in genomic research (see Chapter 37). Primarily these arrays have been developed to meet the growing demands of pharmaceutical research. However, there are few academic centers today that do not have core facilities to prepare low- and high-density arrays to meet the requirements of academic research studying gene expression. 

Dr. C. Kinsland obtained a B.S. in chemistry and a B.A. in French literature from the University of Southwestern Louisiana and an M.S. and Ph.D. in bio-organic chemistry from Cornell University. Since 2001 she has directed the Protein Production and Characterization core facility at Cornell University. 

Table 3 Matching peptide fiagments from MALDI TOF/TOF analysis of Trypsin Digested XlnD analyzed at the Cornell Proteomics and mass spectrometiy core facility at Cornell University in Ithaca, NY. ...

Acknowledgements This work was funded by the DOE Office of the Biomass Program. We would also like to acknowledge the CAFf pretreatment group for providing some of the com stover samples used in this study and Cornell Proteomics and Mass Spectrometry core facility for providing MS data regarding the identification of our purified enzyme. 

Lisa Bibbs Protein and Nucleic Acids Core Facility, The Sciipps Research Institute, La Jolla, California... 

University of Minnesota proteomics core facilities website (http //www.cbs.umn. edu/msp/). 

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