Human Proteome Project

Abbott, A., "Workshop Prepares Ground for Human Proteome Project," Nature, 413, 763 (2001). 

The Human Proteome Project (HUPO, www.hupo.org) initiated the Plasma Proteome Project (PPP) in 2002, and numerous laboratories have contributed to this ambitious project of deciphering all proteins contained in the human plasma. Plasma, the soluble component of the human blood, is believed to harbor thousands of distinct proteins that originate from a variety of cells and tissues through either active secretion or leakage from blood cells or tissues. For the reasons described above, HUPO recommends use of plasma instead of serum, with EDTA (or citrate) for anticoagulation and standardized sample preparation. HUPO proposes combinations of serum depletion, fractionation procedures, and MS/MS technologies, with explicit... 

Humpheey-Smith, 1. (2004). A human proteome project witli a beginning and an end. Proteomics 4, 2519-2521. 

Montelione, G. T. and S. Anderson, Structural genomics keystone for a Human Proteome Project [news]. Nat Struct Biol, 1999. 6(1) p. 11-2. 

Montelione, G.T. and Anderson, S. (1999) Structural genomics keystone for a human proteome project. Nature Struct. Biol. 6 11-12. 

Information flowing from the Human Genome Project is having major influences in fields such as proteomics, bioinformatics, biotechnology, and pharmacogenomics as well as all areas of biology and medicine. 

McKusick VA The anatomy of the human genome a neo-Vesalian basis for medicine in the 21st century. JAMA 2001 286 2289. (The November 14, 2001, issue contains a number of other excellent articles—eg, on clinical proteomics, pharmacogenomics—relating to the Human Genome Project and its impact on medicine.)... 

Riding on the success of the Human Genome Project in cataloging the 30,000 to 40,000 genes of the human genome, the Human Proteome Organization (HUPO http / /www.hupo.org) has set out to determine the entire set of expressed proteins comprising the human proteome(s). As Tyers and Mann... 

The current situation in bioinformatics is characterized by an avalanche of DNA sequences from the human genome project and similar programs and, consequently, an exponential increase in DNA sequences but only a linear increase in protein 3D structures. While multitudes of putative genes have been annotated, up to 90% of all known DNA sequences have no assigned, i.e., experimentally proven, function. From this situation arise the need for interpretation of DNA sequences by information technology, and moreover, analysis of functional genomics and proteomics (see Chapter 15). 

At the start of the twentieth century, biochemistry was dominated by the microbe hunters and replaced later by enzyme and vitamin hunters. In recent decades, biochemistry has been dominated by the gene hunters. The current age of the genome truly represents the most revolutionary advance in the history of biochemistry and medical science. The wealth of information provided by the Human Genome Project lets us anticipate many more exciting discoveries in both genomics and proteomics, areas of study now leading biochemistry in the twenty-first century. 

National Genome Research Network the Human Brain Proteome Project... 

In 2001, the German Federal Ministry of Education and Research (BMBF) initiated the National Genome Research Network (NGFN) as a nation-wide multidisciplinary platform network aiming at the analysis of common human diseases, as well as aging. Within the NGFN the so-called Human Brain Proteome Project (HBPP) focuses on the analysis of the human brain in health and disease. The concept is based on three consecutive steps ... 

Figure 1.2 Adapted workflow of the Human Brain Proteome Project (HBPP) consortium within the second funding period (compare with Figure 1.1).

In the next step of the Human Brain Proteome Project it is planned that identified disease-associated proteins vill be validated using several different techniques. The pathways they play a role in will be analyzed. This will lead to help in understanding the analyzed diseases and to develop diagnostic and/or therapeutic approaches. 

Thiele, H., Apweilee, R., Hamacher, M., Meyer, H. E. (2004). Towards data management of the HUPO Human Brain Proteome Project pilot phase. Proteomics 4, 2361-2362. 

Human Brain Proteome Project -towards an inventory of the brain proteins. BioForum Eur. 8, 28-29. 

Marcus, K., Hultschig, C., Frank, R., Herberg, F. W., Schuchhardt, J., Seitz, H. (2003/2004). Innovative Forschungsansatze im NGFN Verbund The Human Brain Proteome Project HBPP . GenomXPpress. Dec., 5-8. 

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