Plasma proteome project

The Human Proteome Project (HUPO, www.hupo.org) initiated the Plasma Proteome Project (PPP) in 2002, and numerous laboratories have contributed to this ambitious project of deciphering all proteins contained in the human plasma. Plasma, the soluble component of the human blood, is believed to harbor thousands of distinct proteins that originate from a variety of cells and tissues through either active secretion or leakage from blood cells or tissues. For the reasons described above, HUPO recommends use of plasma instead of serum, with EDTA (or citrate) for anticoagulation and standardized sample preparation. HUPO proposes combinations of serum depletion, fractionation procedures, and MS/MS technologies, with explicit... 

Omenn GS, States DJ, Adamski M, Blackwell TW, Menon R, Hermjakob H, et al. Overview of the HUPO plasma proteome project Results from the pilot phase with 35 collaborating laboratories and multiple analytical groups, generating a core dataset of 3020 proteins and a publicly-available database. Proteomics 2005 5(13) 3226-3245. 

Omenn, G. S. (2004a). Advancement of biomarker discovery and validation through the HUPO plasma proteome project. Dis. Markers 20, 131-134. 

In addition to the efforts in the biotech and pharmaceutical industries, a comprehensive, multinational approach to unraveling this biological source has already started with the emergence of the Human Plasma Proteome Project. 

Haab B B, Geierstanger B H, Michailidis G, et al. (2005). Immunoassay and antibody microarray analysis of the HUPO Plasma Proteome Project reference specimens ... 

Human plasma proteome project (HUPO) Human platelets Jurkat T-ceU... 

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