Putative genes

Bryant, J.A. (1988). Putting genes into plants. Plants Today, 1, 23-8. 

Vilchez S, L Moloina, C Ramos, JL Ramos (2000) Proline catabolism by Pseudomonas putida cloning, characterization, and expression of the put genes in the presence of root exudates. J Bacteriol 182 91-99. 

We may also find that Sabatini s reverse transfection method of creating "live cell" microarrays offers even greater advantage for drug discovery and development (Ziauddin and Sabatini, 2001). Their method, relying on arrayed cDNA expression vectors to transform adherent cells, provides localized real-time gene expression analysis of the putative gene product. 

Formation of the Initial Cyclitol. The first steps of FOR production are the same as in the biosynthesis of STR in short, the first step in FOR biosynthesis is postulated to be the formation of a myo-inositol monophosphate (D-myo-inositol-3-phosphate or L-myo-inositol-1-phosphate) via the cellular l-myo-inositol-1-phosphate synthase as in the STR pathway (Ca pathway see Section 2.2.1.2). As in the itr-Att-clusters, no gene for this enzyme has been found in the/or-cluster. As a second step in FOR biosynthesis the dephosphorylation of D-myo-inositol-3-phosphate via an inositolmonophosphate phosphatase has to follow. A putative gene product with this activity is that of the ForA protein (cf. Tables 2.17 and 2.18). The cyclitol is postulated to be first converted via two enzymes, a cluster-encoded myo-inositol 3-dehydrogenase (ForG member of the GFO/IDH/MocA oxidoreductase family) and the L-glutamine icy//o-3-inosose 3-aminotransferase (ketocyclitol aminotransferase I ForS), to icy//o-inosamine (3-deoxy-3-amino- cy/to-mositol). 

The current situation in bioinformatics is characterized by an avalanche of DNA sequences from the human genome project and similar programs and, consequently, an exponential increase in DNA sequences but only a linear increase in protein 3D structures. While multitudes of putative genes have been annotated, up to 90% of all known DNA sequences have no assigned, i.e., experimentally proven, function. From this situation arise the need for interpretation of DNA sequences by information technology, and moreover, analysis of functional genomics and proteomics (see Chapter 15). 

Rice, A. H. and Duffy, J. B. (2001). Characterization of baldspot, a putative gene involved in dorsal-ventral patterning during Drosophila oogenesis. A. Dros. Res. Conf., 42, 661 A. 

The result shows the genes in the query genome along with their functional characterization. As of January 2012, this list contains 777 actual and/or putative genes. 

The sequencing of the chloroeremomycin biosynthesis gene cluster in 1998 allowed the putative gene functions for the assembly of glycopeptide antibiotics to be deduced for the first time. Until now the biosynthesis gene clusters of several other glycopeptide producers have been sequenced baUiimycin Amycolatopsis... 

Cao, J.X., Gershon, P.D., Black, D.N., 1995. Sequence analysis of Hindlll Q2 fragment of capripoxvirus reveals a putative gene encoding a G-protein-coupled chemokine receptor homologue. Virology 209, 207-212,... 

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