Human pituitary proteome

Zhan X, Desiderio DM. 2003. Heterogeneity analysis of the human pituitary proteome. Clin Chem 49 1740. 

Beranova-Giorgiarmi, S., F. Giorgianni, et at, 2002, Analysis of the proteome in the human pituitary. Proteomics 2 534-42. 

MS has been used to characterize 2DGE- and LC-separated human pituitary proteins and DEPs that are related to human pituitary adenomas [2,12,15-17]. PTMs of proteins are also important to study. MS/MS effectively characterizes the PTMs and determines the modified sites. LC-ESI-qlT MS/MS has been used to identify the phosphorylation [18] and nitration sites [19-21] of proteins in the human pituitary proteome. A human pituitary proteome reference database has been established in our laboratory [12,15], many tumor-related proteins were identified [2,17]. And the primary structure and PTMs of pituitary proteins were MS-characterized [2,12,15-21], Those data could contribute to the basic and clinical research studies of human pituitary tumors. Moreover, novel protein chip-based MS technology [22] might eventually be used for the screening, diagnostics, and therapeutics of human pituitary diseases. 

The heterogeneity of human pituitary proteomes is a crucial factor that must be studied in order to accurately validate DEPs between pituitary adenomas and controls because the pituitary adenoma and control tissues are not from the same patient those different sources result in some uncontrolled experimental factors (gender, age, race) that occur within any comparative proteomics study of human pituitary adenomas. Therefore, the heterogeneity of a human pituitary proteome was studied as a function of three important factors—gender, age, and race [16]. 

Desiderio, D.M. and Zhan, X., The human pituitary proteome the characterization of differentially expressed proteins in an adenoma compared to a control. Cell. Mol. Biol., 49, 689-712 (2003). 

Zhan, X. and Desiderio, D.M., Comparative proteomics analysis of human pituitary adenomas current status and future perspectives. Mass Spectrom. Rev., 24, 783-813 (2005). Beranova-Giorgianni, S., Giorgianni, F. and Desiderio, D.M., Analysis of the proteome in the human pituitary. Proteomics, 2, 534-542 (2002). 

Giorgianni, R, Beranova-Giorgianni, S. and Desiderio, D.M., Identification and characterization of phosphorylated proteins in human pituitary. Proteomics, 4, 587-598 (2004). 

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