Human mesenchymal stromal cells

S. Danti, D. D Alessandro, A. Pietrabissa, M. Petrini, S. Berrettini, Development of tissue-engineered substitutes of the ear ossicles PORP-shaped poly(propylene fumarate)-based scaffolds cultured with human mesenchymal stromal cells. J. Biomed. Mater. Res. A 92 (4) (2010) 1343-1356, doi 10.1002/jbm.a.32447. 

Bovine caipometacarpal joint (trabecular bone) Ethylene diamine tetra-acetic acid (EDTA) + sodium dodecyl sulfate (SDS)+DNase and RNase treatments Human adipose-derived stem cells (ASCs), bone marrow-derived human mesenchymal stromal cells (hMSCs), human embryonic stem cells (ESCs) (ceU line H9), human iPSCs (cell lines 11c, 1013A, and BCI) NA de Peppo et al. (2013), Grayson et al. (2008, 2011), Maroltet al. (2012), FrohUch et al. (2010)... 

Human mesenchymal stromal cells (hMSCs) Triton X-lOO-rNH H-t DNase hMSCs Macroporous poly(lactic-co-glycolic acid) (PLGA) scaffold fabricated by gas foaming/ particulate leaching method Rat Decaris et al. (2012a)... 

Giannotti, S., Trombi, L., Bottai, V., Ghilardi, M., D alessandro, D., Danti, S., DelTosso, G., Guido, G., Petrini, M., 2013. Use of autologous human mesenchymal stromal cell/fibrin clot constructs in upper limb non-unions long-term assessment. PLoS One 8, e73893. 

Figure 18.5 Biomimetic scaffold for tympanic membrane replacement based on poly(ethylene oxide terephthalate)-co-poly(butylene terephthalate) and fabricated using additive manufacturing to deposit radial and circular fibers onto an electrospun basement mesh. Zoomed-in micrograph shows human mesenchymal stromal cells stained with methylene blue.

Lange, C., Cakiroglu, E, Spiess, A. N., Cappallo-Obermann, H., Dierlamm, J., and Zander, A. R. 2007. Accelerated and safe expansion of human mesenchymal stromal cells in animal serum-free medium for transplantation and regenerative medicine. / Cell Physiol, 213,18-26. 

R. Schafer, R. Kehlbach, M. Muller, et a/., Labeling of human mesenchymal stromal cells with superparamagnetic iron oxide leads to adecrease in migration capacity and colony formation abil-ity Cytotherapy2009, vol. 11, pp. 68-78. 

Rifas L. T-cell cytokine induction of BMP-2 regulates human mesenchymal stromal cell differentiation and mineralization. J Cell Biochem 2006 July 1 98(4) 706-14. 

P. J. 2009. Priming integrin a5 promotes human mesenchymal stromal cell osteoblast differentiation and osteogenesis. Proc Natl Acad Sci USA, 106,18587-18591. 

Bartosh TJ, Ylostalo JH, Mohammadipoor A, Bazhanov N, Coble K, Claypool K, Lee RH, Choi H, Prockop DJ. 2010. Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties. Proc Natl Acad Sci USA 107(31) 13724-9. 

Schop D, van Dijkhuizen-Radersma R, Borgart E, Janssen FW, Rozemuller H, Prins HJ, de Bruijn JD. 2010. Expansion of human mesenchymal stromal cells on micro carriers Growth and metabolism. / Tissue Eng Regen Med 4(2) 131-40. 

GMP-COMPLIANT PROPAGATION OF HUMAN MULTIPOTENT MESENCHYMAL STROMAL CELLS... 

Acronyms and Definitions Mesenchymal Stromal Cells Somatic Stem Cell Therapy Good Manufacturing Practice Cell-Based Medicinal Products Human Platelet Lysate... 

Muller, I., Kordowich, S., Holzwarth, C., et al. (2006), Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM, Cytotherapy, 8(5), 437-444. 

Christina Bartmann, Medical University of Graz, Graz, Austria, GMP-Compliant Propagation of Human Multipotent Mesenchymal Stromal Cells... 

Effects of chemotherapeutic agents on the proliferation, differentiation, and stromal support capacity of human mesenchymal stem cells Archambault, M.P., Howard, L., Butterfield, A.M., Peter, S.J. (2001). Blood, 98 11 Suppl 2 Abs 4235. 

Seih F P, Prewitz M, Werner C and Bomhauser M (2009), Matrix elasticity regulates the secretory profile of human bone marrow-derived multipotent mesenchymal stromal cells (mscs) , Biochem Biophys Res Commun, 389, 663-7. 

Wang, L., et al. 2010. Osteogenic differentiation of human umbilical cord mesenchymal stromal cells in polyglycolic acid scaffolds. Tissue Engineering Part A 16(6) 1937-1948. 

Salerno et al. cocultured human hepatocytes with human umbilical vein ECs (HUVECs) on a polyetheretherketone PU membrane to promote vascularization of an in vitro engineered liver tissue [102]. There was improved hepatocyte function and the formation of luminal structures occurred within 3 days of coculture on the PU versus hepatocyte-only samples [102]. Another study demonstrated that coculturing bone marrow-derived mesenchymal stromal cells with hepatocytes greatly reduced the secretion of stress enzymes by the hepatocytes and that the cells attached better to nanoflber PU versus unstructured PU [103]. 

Huang, Z., Nooeaid, P., KohL B., Roether, J.A., Schubert, D.W., Meier, C., Boccaccini, A.R., Godkin, O., Ertel, W., Arens, S., Schulze-Tanzil, G., 2015. Chondrogenesis of human bone marrow mesenchymal stromal cells in highly porous alginate-foams supplemented with chondroitin sulfate. Mater. Sci. Eng. C Mater. Biol. Appl. 50, 160—172. 

Covas, D. T., Panepucci, R. A., Fontes, A. M., Silva, W. A., Jr., Orellana, M. D., Freitas, M. C., Neder, L., Santos, A. R., Peres, L. C., Jamur, M. C., and Zago, M. A. 2008. Multipotent mesenchymal stromal cells obtained from diverse human tissues share functional properties and gene-expression profile with CD146-I- perivascular cells and fibroblasts. Exp Hematol, 36, 642-54. 

Muller, I., Kordowich, S., Holzwarth, C., Spano, C., Isensee, G., Staiber, A., Viebahn, S., Gieseke, R, Langer, H., Gawaz, M. R, Horwitz, E. M., Conte, R, Handgretinger, R., and Dominici, M. 2006. Animal serum-free culture conditions for isolation and expansion of multipotent mesenchymal stromal cells from human BM. Cytotherapy, 8,437-44. 

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