Horse digestion

Figure 6 High-speed tryptic fingerprint. Horse cytochrome c was digested with trypsin and the peptide chromatographed in acetonitrile water 0.1% TFA at various temperatures and flow rates on a 15 x 0.2-cm PS-DVB column packed with 3-p, 300-A particles, (a) 26°C and 0.5 ml/min. (b) 42°C and 0.7 ml/min. (c) 70°C and 1.1 ml/min. Detection at 220 nm. Note that the resolution rises with the speed of separation.89 (From Swadesh, ]., BioTechniques, 9, 626, 1990. With permission.)...

Effect of substrate concentration. In the following experiments the cholinesterase activities were measured by a continuous titration method. The digest of acetylcholine and horse-serum cholinesterase (total vol. 10 ml.), containing bromothymol blue and 0-0002 m phosphate, was titrated with 0-01 n NaOH to maintain the pH at 7-4. The titrations, which were carried out at 20°, were linear over a period of 10-15 min. The velocity was expressed as ml. 0-01 n NaOH/5 min. under the conditions used, it was proportional to the enzyme concentration. When an inhibitor was added, this was equilibrated with the enzyme, etc., for 5 min. at 20° before adding the substrate contained in a volume of 1 ml. 

Bertone, A.L., Ralston, S.L. and Stashak, T.S. (1989b) Fibre digestion and voluntary intake in horses after adaptation to extensive large colon resection. American Journal of Veterinary Research 50, 1628-1632. 

Machelboeuf, D., Marangi, M., Poncet, C. and Martin-Rosset, W. (1995) Study of nitrogen digestion from different hays by the mobile bag technique in horses. Annales de Zootechnie 44, Supplement 219. 

Kidney and the digestive system of the horse mussel Cd, Cu, Fe, Pb, Mg and Mn FAAS, GFAAS, Ultracentrifugation 47)... 

Aesculus chinensis L. A. indica Colebr. A. hippocastanum L. Sha Lou Zi (Horse chestnut) (ripe fruit) Protoescigenine, escigenin, aescine, flavonoid glycosides, aesculine, albumin, fatty oils, amylose, oligosaccharides.33 450 Promote circulation, relieve epigastrium pain, promote digestion. 

IgG2), whereas bovine, sheep, and horse antibodies are somewhat resistant to digestion and require a full 48 h. 

Proteolytic digestive enzyme activity in sluggish Black Sea fish (e.g. toad goby, scorpion fish) has been found to be equal to and sometimes greater than that of the active fish (e.g. horse-mackerel, annular bream, pickerel). The aminolytic activity of fish of low mobility is also greater (Ugolev and Kuzmina, 1993). It will further be shown in Chapter 5 that the consumption and assimilation of food occur more efficiently in sluggish than in active fish. 

On-line protein digestion has been achieved by trypsin adsorbed in a porous PVDF (poly [vinylidene fluoride]) membrane (0.45-pm pore) coupled in a PDMS chip. In this way, on-chip protein digestion and subsequent MS analysis have been carried out for horse heart cytochrome c [817]. 

Transfer capillary, glass chip EOF ESI-ITMS Tryptic digest of horse apomyoglobin (7.4 nM), yeast proteins Trypsin Non-chip gradient frontal CEC separation C18 column 803... 

Enzymatic microreactors (7.5 nL) have been fabricated in the microfluidic chip to prepare the tryptic digest of equine (horse) myoglobin (14.2 pmol/p.L)... 

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