Homogeneous cell preparation

A new technique to prepare nC60 (fullerene sonication in methanol) has been used, but in this case the cluster sizes are not homogeneous. This preparation permeates the cell membrane of normal and malignant breast epithelial cells (MCF10A and MDA MB 231 or MDA MB 435, respectively) without interfering with cellular events. Also the incubation with high concentration (200ppm) does not adversely impact cell proliferation (Levi et al., 2006). 

A sonic homogenate is prepared of a fibroblast cell pellet in 250-pl distilled water using a tip sonificator, followed by centrifugation for 10 min at 10,000 rpm (about... 

Generally, the concentration of mAbs in the interstitial fluid is rather small, with reported serum tissue ratios in biopsy samples ranging from 2 to 10. However, as this sampling technique normally uses homogenization during preparation, only a mixed concentration across all different cell types and fluids of the entire tissue is provided, and the ratios will most likely be an overestimation of the real situation. 

The source of enzyme was normal human melanocytes grown in cultured media. Homogenates were prepared by subjecting washed cells to sonication or homogenization in a Potter tube. In their discussion Smit et al. make a... 

Immunofluorescent detection of TS protein was done with the use of monoclonal antibodies, developed by in vivo immunization of Balb/c mice with homogeneous recombinant rat hepatoma TS protein as an antigen. The specific anti-rat TS antibodies recognized also T. spiralis TS, as indicated by cross-reactivity on Western blot. Localization of the enzyme was based on analysis of pictures collected by confocal microscopy. Two types of T spiralis muscle larvae preparations were studied muscle larvae isolated from mouse muscles by a procedure destroying nurse cells and muscle larvae remaining in nurse cells, isolated as an intact nurse cell preparation. 

The most prevalent catalysts are homogeneous metal complexes mostly with chiral diphosphine ligands, isolated enzymes and whole cell preparations, whereas... 

For most of these latter compounds, the estimated dissociation constants in whole cell preparations were at least two orders of magnitude greater than the values obtained in brain homogenates (Table 2.6). In particular, the antidepressant drugs appear to be rather weak H2-receptor antagonists in intact cell systems and this result weakens the argument that these compounds derive their clinical efficacy from blockade of H2-receptors in mammalian brain. 

S2, S8). Others have reported that adenosine inhibits adenylate cyclase from brain, adipocytes, and lung tissue (FI, M6, Wl). Brain tissues of most animals have very little adenosine deaminase. In other tissues the role of breakdown products must be further explored. The fact that cyclase in intact slices is stimulated while that of broken-cell preparations is inhibited may be related to the so-called latent adenosine deaminase that is mitochondria-bound and inactive in assays of adenosine deaminase in homogenates (M16). 

Cell Synchrony and Collection of Mitotic Cells Preparation of Cell-Free Homogenates Nuclear Reassembly... 

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