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Hollow fibre culture

Von Wedel RJ (1987) Mass culture of mouse and human hybridoma cells in hollow-fibre culture. In Seaver SS (ed.) Commercial Production of Monoclonal Antibodies. A Guide for Scale Up, pp. 159-173. Marcel Dekker, New York. [Pg.239]

Hanak, J.A.J., Combridge B.S., and Kings-land, S.L. (1995) Human therapeutic monoclonal Anti-D antibody produced in longterm hollow-fibre culture. In Beuvery, E.C. et al. (Eds) Animal Cell Technology Developments towards the 21st century. Kluwer Academic Publishers, pp. 149—153. [Pg.757]

Klerx, J. P. A M., Jansen Verplanke, C., Blonk, C. G, and Twaalfhoven, L. C (1988) In vitro production of monoclonal antibodies under serum-free conditions using a compact and inexpensive hollow fibre cell culture unit. J Immunol Methods 111, 179-188. [Pg.56]

Scale-up of cell cultures makes use of suspension cultures (erythropoietic cells or microcarriers) or, less often use of capillary beds (hollow fibre systems or glass bead columns), but these suffer from the same disadvantages seen with smaller scale cultures ( 3.4.4). In particular, nutrients are depleted as the medium flows through long columns or beds and high rates of flow coupled with recirculation are often employed. Nevertheless, Organon have used a hollow fibre dialysis system for production of monoclonal antibodies (Schonherr et al., 1985). Invitron s hollow fibre system has been used to produce cell conditioned media and the Cell-Pharm System (Jencons Ltd. Appendix 3) will produce up to 20 g cell secreted product per month. [Pg.56]

Extracorporeal Hver assistance device (ELAD) Attention has recently focused on temporarily replacing the liver function with hepatocytes which have been cultured in the extracapillary space of a cellulose-acetate hollow-fibre unit. Each unit contains ca. 200 g C3A cells, an amount which is necessary for successful perfusion. ELAD has proved efficacious in clinical use. (106)... [Pg.386]

If is obtained from experiments in stirred suspension or microcarrier cultures, as described above, then, it may be possible to estimate from Q- lqu- However, care must be exercised because can be altered by changes in the culture environment. For example, qy for cells immobilized in agarose beads or hollow fibres may be different from qy for the same cells grown in the same medium in a stirred suspension reactor (Shirai et al, 1988 Wohlpart et al, 1991). In addition, qy may change over time due to changes in cell, nutrient and byproduct concentrations. Analysis of cell density and immobilization effects is complicated by the presence of nutrient concentration gradients. However, stirred vessels with cells immobilized... [Pg.155]

The most common cell culture systems developed for pilot- and commercial-scale production of monoclonal antibodies (MAbs) are hollow-fibre and ceramic matrix modules, stirred bioreactors and airlift fermenters. These systems allow cultivation of cells in batch, fed-batch, continuous or perfusion mode. The selection of a culture system and culture mode for the large-scale production of a particular MAb should take into account the growth and antibody-production characteristics of the particular hybridoma line. This module therefore presents an overview of the important characteristics of these systems. Detailed descriptions with accompanying results and a large collection of cited literature are given elsewhere (Seaver, 1987 Mizrahi, 1989 sections 5.1 and 5.9). [Pg.235]

A great deal of effort has gone into the development of high-density immobilized perfusion culture systems, which can be operated continuously. The limitation with many of these systems (e.g. hollow fibre) is that, whilst they achieve very high unit cell density (typically 10 mH), they do not scale up well volumetrically. This limitation has been overcome by the use of porous carrier immobilization techniques, where high unit cell density can be combined with good volumetric scale-up potential and long-term continuous operation. [Pg.279]

The Tecnomouse (29) is a hollow fibre bioreactor containing up to five flat culture cassettes containing hollow fibres surrounded by a sihcone membrane that gives uniform otygenation and nutrient supply of the culture and ensures homogeneity within the culture. The system comprises a control unit (media supply), a gas and medium supply unit, and the five culture cassettes. [Pg.138]

If a low-secreting cell line is the best available and the antibody is important, the only option is to grow a large quantity of cells and to concentrate the culture supernatant before further processing. These objectives can both be accomplished in a hollow fibre fermenter. Alternatively, supernatant from conventional cultures can be concentrated in a variety of devices. Hollow fibre kidney dialysis cartridges are particularly convenient, being reliable, free from endotoxin, easy to use, and cheap. [Pg.151]

Concentration of culture supernatant using a hollow fibre device... [Pg.151]


See other pages where Hollow fibre culture is mentioned: [Pg.431]    [Pg.128]    [Pg.134]    [Pg.386]    [Pg.222]    [Pg.235]    [Pg.60]    [Pg.134]    [Pg.137]    [Pg.138]    [Pg.138]    [Pg.145]    [Pg.134]    [Pg.137]    [Pg.138]    [Pg.138]    [Pg.145]    [Pg.151]   
See also in sourсe #XX -- [ Pg.10 , Pg.226 , Pg.235 ]




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