Hollow fibre Acusyst culture

The Tecnomouse (29) is a hollow fibre bioreactor containing up to five flat culture cassettes containing hollow fibres surrounded by a sihcone membrane that gives uniform otygenation and nutrient supply of the culture and ensures homogeneity within the culture. The system comprises a control unit (media supply), a gas and medium supply unit, and the five culture cassettes. 

The culture is initiated with cells from 3 x 225 cm flasks (5 x 10 ) and grows to 5 X 10 cells. The continuous mediiun supply is controllable and program- 

Medium IMDM 1%PCS IMDM S. Free IMDM S. Free  

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