Histidine -tagged proteins

Janknecht, R., G.de Martynoff, J.Lou, R. A.Hipskind, A.Norandeim and H.G.Stunnenberg. (1991) Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia vims. Proc Natl Acad Sci USA, 88, 8972-8976. 

Crofcheck, C., Loiselle, M Weekley, J Maiti, 1., Pattanaik, S., Bummer, PM., and Jay, M. (2003). Histidine tagged protein recovery from tobacco extract by foam fractionation. Biotechnol. Prog. 19(2) 680-682. 

Protease inhibitor cocktail (for histidine-tagged proteins) (Sigma-Aldrich, St. Louis, MO). 

Wasowicz, M., Viswanathan, S., Dvomyk, A., Grzelak, K., Kludkiewicz, B., and Radecka, H. 2008. Comparison of electrochemical immunosensors based on gold nano materials and immunoblot techniques for detection of histidine-tagged proteins in culture medium. Biosens Bioelec 24 284—289. 

Lige B, Ma S, Zhao D, Van Huystee RB. Cationic plant peroxidase Expression and characterization in transgenic tobacco and purification of the histidine-tagged protein. Plant Sci 1998 136(2) 159—168. 

Greiner Aminosilane Aldehyde and Epoxy Nickel Oligonucleotides and PCR products Amino-modified oligonucleotides and PCR products, or proteins Histidine-tagged proteins www.greinerbiooneinc.com... 

Nieba L., Nieba-Axmann S. E., Rersson A., Hamalainen M., Edebratt F., Hansson A., Lidholm J., Magnusson K., Karlsson A. F., and Rliickthun A., BIAcore analysis of histidine-tagged proteins using a chelating NTA sensor chip, Anal. Biochem., 252, 217-228, 1997. 

This protocol describes the preparation and attachment of histidine-tagged proteins to the bilayers containing Ni-NTA in the phospholipid headgroups. The main advantage of this technique is that the genetic fusion of the histidine tag with the protein results in a predictable orientation of the protein on the bilayer and maintains protein s natural stoichiometry. Alternatively, proteins can also be attached to lipid bilayers covalently, or by a streptavidin-biotin linkage, but these techniques can result in random orientation and multimerization of the proteins. 

The protocol here outlines production of a secreted recombinant protein in mammalian cells and purification via the histidine tag, but other methods of preparation of pure histidine-tagged proteins should work equally well. 

A different approach to achieving selective adsorption of engineered oligo-histidine-tagged proteins with minimal interference of host cell proteins involves tailor-made chelating supports with very short spacer arms and low surface density of chelating groupst l... 

Clemmitt R.H. and Chase H.A. 2000. Facilitated downstream processing of a histidine-tagged protein from unclarified E. coli homogenates using immobilized metal affinity expanded-bed adsorption, Biotechnol. Bioeng., 67, 206. 

Figure 10.18 Protocol for CPNW fabrication. (Reprinted with permission from Biosensors and Bioelectronics, Label-free detection of cupric ions and histidine-tagged proteins using single poly(pyrrole)-NTA chelator conducting polymer nanotube chemiresistive sensor by C. L. Aravinda, S. Cosnier, W. Chen et ai, 24, 5. Copyright (2009) Elsevier Ltd)...

Importin a is readily prepared in active form and in relatively large quantities as a histidine-tagged protein in Escherichia coli (Weis et ai, 1995). We have... 

Wilson-Kubalek et al. also produced specifically and nonspecifically functionalized unilamellar lipid tubules by using mixtures of a tubule-forming galactosylceramide and various charged or derivatized lipids [166]. Thus, nickel-doped lipids allowed the helical crystallization of histidine-tagged proteins. The authors also reproduced the helical crystallization of streptavidin. They even obtained helical arrays of relatively small proteins, such as actin and annexin, as well as large macromolecules, such as RNA polymerase (Fig. 21). 

Recombinant human MxA is expressed in E. coli as an N-terminal histidine-tagged protein using the pQE-9 vector system (Qiagen, Hilden, Germany), as described (Kochs et al., 2002a Richter et al., 1995). 

---