Vaccinia vims

Replacement of the sulfur atom in the thiosemicarba2one moiety by an oxygen atom leads to a loss of antiviral activity. Methisa2one has no significant effect on vaccinia vims DNA synthesis (14) but seems to inhibit late protein synthesis by a mechanism that remains to be elucidated. 

Topical apphcation of ara-HxMP, C2QH23N40gP, significantly inhibited the development of keratitis-induced HSV-1, HSV-2, or vaccinia vims in the eyes of rabbits. Ara-HxMP also significantly controlled the development of HSV-1 or vaccinia viral-induced encephaUtis in mice and was also active in preventing equine abortion viral deaths in hamsters. Clinical trials with ara-HxMP have not yet been reported. 

The cychc phosphonate of ( -HPMPA, [( -cHPMPA [106941-26-8], C H 2N50 P, 28] also is active against HSV-1 and HSV-2 infection in mice. Both (27) and (28) are reported to be efficacious against adenovims, vaccinia vims, as weU as variceUa-2oster vims infections (58). 

Janknecht, R., G.de Martynoff, J.Lou, R. A.Hipskind, A.Norandeim and H.G.Stunnenberg. (1991) Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia vims. Proc Natl Acad Sci USA, 88, 8972-8976. 

Environmental Influences on the Relative Stability of Baculoviruses and Vaccinia Vims A Review... 

Harper, J.M.M., Parsonage, M.T., Pelham, H.R.B., and Darby, G., 1978. Heat inactivation of vaccinia vims particle-associated functions properties of heated particles in vivo and in vitro. J. Virol., 26 646-659. 

Kampf, G., Steinmann, J., and Rabenau, H., 2007. Suitability of vaccinia vims and bovine viral diarrhea vims (BVDV) for determining activities of three commonly-used alcohol-based hand mbs against enveloped vimses. BMC Infect. Dis., 7 5. 

Kligler, I.J., and Bemkopf, H., 1937. Inactivation of vaccinia vims by ascorbic acid and glutathione. Nature, Lond., 139 965. 

McCrea, J.F., Preiss, J.W., and O Loughlin, J., 1960. Physical studies on pox vimses. I. Inactivation of vaccinia vims infectivity with low-energy electrons. Biophy. J., 1 43-53. 

Remington, K.M., Trejo, S.R., Buczynski, G., Li, H., Osheroff, W.P., Brown, J.P.,Renfrew, H., Reynolds, R., and Pifat, D.Y., 2004. Inactivation of west Nile vims, vaccinia vims and viral surrogates for relevant and emergent viral pathogens in plasma-derived products. Vox Sang., B7 10-18. 

A. Domi and B. Moss, Cloning the vaccinia vims genome as a bacterial artificial chromosome in Escherichia coli and recovery of infectious vims in mammalian cells, Proceedings of the National Academy of Sciences USA, 99, 2002, 12415-20. 

B. Moss, Vaccinia vims expression vector a new tool for immunologists, Immunology Today, 6, 1985, 243-5 J. A. McCart et al., Systemic cancer therapy with a tumor-selective vaccinia vims mutant lacking thymidine kinase and vaccinia growth factor, Cancer Research, 61, 2001, 8751-7. 

DNA topoisomerases can be classified into three evolutionary independent families type lA, type IB and type II. The Escherichia coli topoisomerase I and topoisomerase III, Saccharo-myces cereviaae mpoisometase III and reverse gyrase belong to the type lA or type 1-5 sub-family as the protein link is to a 5 phosphate in the DNA. The prototype of type IB or 1-3 enzymes are found in all eukaryotes and also in vaccinia vims topoisomerase I where the protein b attached to a 3 phosphate. Thougli essentially similar in their action, these enzymes have a broader specificity than that of atU enzyme. Despite the differences in the mechanbm and specificity between the bacterial and eukaryotic en unes, the yeast DNA topoisomerase I has... 

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