Oligonucleotides amino-modified

Depending on the size of the probe, either covalent (Figures 10 and 11) or electrostatic immobilization (Figure 12) may be preferred. In general, oligonucleotides and DNA fragments of approximately 20 to 70 bases are amino-modified and bound covalently to the chip surface. Complete or partially complementary DNA of up to 5000 nucleotide bases is bound to the chip by electrostatic adsorption. 

Fig. 8 Covalent attachment of amine-terminated oligonucleotides to amino-modified (APS) supports via a PDITC linker...

To arrange AuNPs into a monolayer, Simon and coworkers [74] reported a simple protocol via the oligonucleotides complementary immobiUzation. 5 -Amino-modified oligonucleotide was immobilized on the substrate surface first, and then, 15 nm gold particles modified with thiolated DNA oligomers were coupled to the surface through DNA base pairing. The resulted nanoparticle monolayers were demonstrated to have a thermally activated conductivity. A similar approach has been used to insert a liposome into a DNA chip [75] (Fig. lib). 

Telechem Primary amine Primary aldehyde Oligonucleotides and PCR products Amino-modified oligonucleotides and PCR products, or proteins www.arrayit.com... 

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