High-throughput protein crystallography

Bemtson, A., Stojanoff, V. and Takai, H. (2(X)3). Application of a neural network in high-throughput protein crystallography. /. Synchrotron Rud. 10,445-449. 

Thus, it is but natural to incorporate the structural information from the target to bias compound selection prior to the experimental testing (9, 17, 18). With the advent of high-throughput protein crystallography (19), structural genomics... 

The demand for a high-throughput protein crystallography (HTPC) is driven by the need to understand exactly how proteins bind to their ligands (BlundeU et al, 2002) in meeting... 

Wiithrich K (2010) NMR in a crystallography-based high-throughput protein structure-determination environment. Acta Crystallogr F-Struct Biol Cryst Commun 66 1365-1366... 

Protein-protein interaction pathways and cell signalling networks, high-throughput protein structural studies using mass spectrometry, nuclear magnetic resonance, X-ray crystallography are also expanding fields. 

Kuhn P, Wilson K, Patch MG, et al. The genesis of high-throughput structure-based drug discovery using protein crystallography. Curr Opin Chem Biol 2002 6 704-10. 

A fully automated protein crystallography beamline at a third-generation synchrotron source can screen several hundred crystals daily. Automatic evaluation of the diffraction images to ascertain crystal quality is, therefore, a critical step for high-throughput data acquisition. Evaluation of each image requires software that mimics the traditional visual assessment of crystal quality. 

Any high-throughput system for protein crystallography requires efficient processes for converting measured diffraction images into experimental electron density maps and structures. Chapters 6 to IT and T 3 cover the various approaches to data analysis in considerable depth. 

Jhoti, H. (2004). High-throughput crystallography. In Protein Crystallography in Drug Discovery, Babine, R. E. and Abdel-Meguid, S.S., eds. WUey-VCH Verlag, Weinheim. 

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