High throughput assays

We developed an ampUfied /uminescence /Proximity /zomogeneous assay (AlphaScreen) for in vitro screening. This assay detects the proximity of donor 

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With this focus on CYP and fiver metabolism, most companies have established high throughput assays to measure compound stability in the presence of human (or preclinical species) fiver microsomes [49]. Disappearance of starting compound from an incubation with microsomes is monitored. Measurement at a single time point enables a rank-ordering of compounds for stability based on percent of parent compound remaining acquisition of data at multiple time points allows determination of half-life, intrinsic clearance, and extrapolation to a predicted in vivo clearance [50]. 

Introduces microparallel liquid chromatography (LC), ADME/PK high-throughput assay, MS-based proteomics, and the advances of capillary and nano-HPLC technology... 

High-throughput assay 10.000- 100.000 Enzymatic assays based on Fluorescence and luciferase [44]... 

Ultra High-throughput assay > 100.0000 Transcription factor Kruppel-like factor 5 (KLF5) [45]... 

High-throughput assay of bile acids is technically demanding and expensive. Combined with high intra- and inter-individual variability in stool bile acid levels, it is unlikely that bile acid measurement will find a role as a biomarker of future colorectal neoplastic risk. 

Feng BY, Shelat A, Doman TN, et al. (2005) High-throughput assays of promiscuous inhibitors. Nat. Chem. Biol. 1 146-148. 

Plasma protein binding is also an important parameter in the pharmacokinetic field. Frontal analysis combined with capillary zone electrophoresis (CZE-FA) (67-69) is a powerful technique for high-throughput assay, because it is relatively rapid and easy to automate, in comparison with conventional methods such as dialysis, ultrafiltration, and ultracentrifugation. Recently, we introduced the EKC approach with ionic CDs to frontal analysis for anionic drugs that cannot be analyzed by conventional CZE-FA (70). In this approach, ionic CDs work as an EKC pseudostationary not for proteins but for small solutes. 

Key words High-throughput assay. Two-dimensional electrophoresis. Automation, System, Simultaneous detection. Protein... 

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