High-throughput screening assays

Table 8.2 shows the range of possible 71 values, with comments on their meaning in terms of high-throughput screening assays. 

Macarron R, Hertzberg RP (2002) Design and implementation of high throughput screening assays. Methods Mol Biol 190 1-29... 

Zhang JH, Chung TD, Oldenburg KR (1999) A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 4(2) 67-73... 

Andexer, J., Guterl, J.-K., Pohl, M. and Eggert, T. (2006) A high-throughput screening assay for hydroxynitrile lyase activity. Chemical Communications, 4201 4203. 

Macarron, R., and Hertzberg, R. P. (2002). Design and Implementation of High Throughput Screening Assays. Humana Press, Totowa, NJ. 

Compared to in vivo studies, the Caco-2 model substantially increases the speed at which absorption potential can be estimated and reduces the amount of drug substance needed. However, manually performed assays are still too slow and labor intensive compared to biological high-throughput screening assays. Caco-2 cells take about 3 weeks to form monolayers of fully differentiated cells. At this point, Caco-2 monolayers are used to evaluate absorption potential under a variety of permeability protocols. In order to further expedite the process of absorption potential assessment, efforts have been made to increase the throughput of Caco-2 transport experiments. 

Powell, K.D., Fitzgerald, M.C. High-throughput screening assay for the tunable selection of protein ligands. 

Opaque white wall, clear bottom 96-well tissue culmre plates to be used with a luminescent cell viability reagent suitable for high-throughput screening assays. 

Archer JR (2004) History evolution, and trends in compound management for high throughput screening. ASSAY Drug Dev Technol 2 675-681... 

Zhang X. D, (2007) A pair of new statistical parameters for quality control in RNA interference high-throughput screening assays. Genomics 89 552-561... 

Li Q, Chen C, Kapadia A et al (2011) 3D models of epithelial-mesenchymal transition in breast cancer metastasis high-throughput screening assay development, validation, and pilot screen. J Biomol Screen 16 141-154... 

Development of a High-Throughput Screening Assay for Carboligase Activity... 

D.S. (2007) High-throughput screening assays for the identification of chemical probes. Nat. Chem. Biol., 3 (8), 466-479. 

DESIGNING A HIGH-THROUGHPUT SCREENING ASSAY FOR TESTING TRANSDERMAL FORMULATIONS... 

MH Pausch. G protein-coupled receptors in Saccharomyces cerevisiae. high-throughput screening assays for drug discovery. Trends Biotechnol 15 487-494, 1997. 

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