Cell-Based High-Throughput Assays

Cronk D (2001) Cell-based high-throughput screening of ion channels using FLIPR and VIPR membrane potential assay technology. Presented at 7th annual conference of Society for Biomolecular Screening, Baltimore, MD, 10-13 September 2001. SBS, Danbury, Abstract no 119... 

Nuclear magnetic resonance (NMR) measurements are usually considered to be slow processes, but recent advances in the design of flow-through NMR cells have allowed the method to be applied in combinatorial chemistry 97). These technological improvements were applied to the development of two NMR-based high-throughput ee assays for evaluating the products of enzyme- or transition metal-catalyzed reactions 98). 

The process of identifying new chemical entities (NCEs) often involves the use of cell-based or biochemical assays by which the effects of new compounds on a specific target of interest are monitored. Transformed cell lines have been the traditional mainstays of assay development since the beginning of high-throughput screening (HTS) in the early 1980s. 

Pedersen, C. Krog-Jensen, M.M. Rosenkilde, L. Pagliaro, High content screening for G protein-coupled receptors using cell-based protein translocation assays, Comb. Chem. High Throughput Screen. 2005, 8, 301-309. 

Kemnitzer, W. et al. Discovery of 4-Aryl-477-chromenes as a new series of apoptosis inducers using a cell- and caspase-based high-throughput screening assay. 1. Structure-activity relationships of the 4-aryl group. J. Med. Chem. 2004, 47, 6299-6310. 

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