High performance liquid chromatography column packing material

Unfortunately, most column and sorbent manufacturers do not develop column packing materials mainly for SEC work, but for the bigger high-performance liquid chromatography (HPEC) market. However, there are many important differences to consider when designing packings for different modes of chroma-... 

High-performance liquid chromatography (HPLC) is a fast technique that, with high precision and specificity, separates mixtures into individual ingredients. Used as a routine procedure, it has several advantages It can be completely automated sample cleanup and preparation are simple and the reproducibility of the packing material means that the analytical conditions remain the same for a new column. 

In this variation of high performance liquid chromatography (HPLC), the columns are packed with material that will hold back molecules dependent on the molecular size. Using the correct solvent system and column types, the molecular weight and distribution of the polyols can be determined. Standard samples are needed to calibrate the system. The molecular weight distribution of prepolymers can also be determined. This will enable an estimation to be made of the number of soft segment chains there are. 

Various workers [23-32] have studied the application of high performance liquid chromatography to the determination of PAHs in water samples. Hagenmaier et al. [31] used a reversed phase high-pressure liquid chromatography procedure for the determination of trace amounts of polycyclic aromatic hydrocarbons in water. Different column packing materials were tested, in conjunction with non polar stationary phases of various polarities, for separation efficiency, detection limits and long-term stability. The method was suitable for concentrations as low as 2ng L 1 in a IL sample. Compounds... 

Arsenic species were preconcentrated on Zipax, a pellicular anion exchange material and separated on a column packed with high performance liquid chromatography grade strong anion exchange resin, then continuously reduced with sodium tetrahydroborate and detected by atomic absorption spectrometiy. Detection limits were 2ng for arsenite, arsenate and monomethylarsinate and lng for dimethylarsonate. 

High performance liquid chromatography on a small bore column packed with microparticulate silica based ion exchange material has been used [317] to determine down to 0.01 mg L 1 nitrate in water without interference from other ions associated with potable, pond, river and stream water. 

The column is the only device in the high-performance liquid chromatography (HPLC) system which actually separates an injected mixture. Column packing materials are the media producing the separation, and properties of this media are of primary importance for successful separations. 

K. Murayama, H. Nakamura, T. Nakajima, K. Takahashi, and A. Yoshida, Preparation and evaluation of octadecyl titania as column-packing material for high-performance liquid chromatography, Microchem. J. 49 (1994), 362-367. 

---