High performance liquid chromatography capillary electrophoresis compare

LEE B L and ONG c N (2000) Comparative analysis of tea catechins and theaflavins by high-performance liquid chromatography and capillary electrophoresis , J Chromatog A, 881 (1-2), 439-47. 

In the last decade, capillary electrophoresis (CE) has become one of the most powerful and conceptually simple separation techniques for the analysis of complex mixtures. The main reasons are its high resolution, relatively short analysis times, and low operational cost when compared to high-performance liquid chromatography (HPLC). The ability to analyze ultrasmall volume samples in the picoliter-to-nanoliter ranges makes it an ideal analytical method for extremely volume-limited biological microenvironments. 

Lee BL, New AL, Ong CN (1997) Comparative analysis of conjugated bile acids in human serum using high-performance liquid chromatography and capillary electrophoresis. J Chro-matogr Biomed Sci Appl 704 35-42... 

Steuer et al. compared supercritical fluid chromatography with capillary zone electrophoresis (CZE) and high-performance liquid chromatography (HPLC) for its application in pharmaceutical analysis [24]. Efficiency, performance, sensitivity, optimization, sample preparation, ease of method development, technical capabilities, and orthogonality of the information were the parameters studied. They concluded that SFC is ideal for moderately polar compounds, such as excipients, for which mass detection is required. 

As for all synthetic products to be tested in biological systems, a careful analytical characterization of peptide libraries is crucial in order to confirm their identity and establish their quality. Compared to individual peptides, however, the analysis of peptide libraries is complicated due to the fact that the peptides are either bound to a solid support or arranged in highly complex mixtures. This poses certain restrictions on which analytical methods can be used to characterize combinatorial libraries. For example, analytical methods that are based on the separation of product components, such as high performance liquid chromatography (HPLC) and capillary electrophoresis (CE), are only of limited use for the analysis of peptide libraries, in particular of those made up of complex nnixtures (>100 peptides per mixture). The analytical methods beneficially applicable to peptide libraries include amino acid analysis, mass spectrometry, and sequencing. 

Terzuoli, L. Porcelh, B. Setacci, C. Giubbolini, M. Cinci, G. Carlucci, F. Pagani, R. MarineUo, E. Comparative determination of purine compounds in carotid plaque by capillary zone electrophoresis and high-performance liquid chromatography. J. Chromatogr., B 1999, 728, 185-192. 

MLL de Moraes, B Polakiewicz, MF Mattua, MFM Tavares. Comparative evaluation of capillary electrophoresis and high-performance liquid chromatography for... 

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