High content screening types

High-throughput automated imaging systems, also sometimes referred to as high content screening (HCS) systems, enable the study of specific cell types within a mixed population of cells (Giuliano, 1997). Some primary cell types are dependent on interactions with other cell types, making their cultivation in isolation impossible, at least at the present time. Even when primary cells are not dependent on interactions with other cell types, primary cell preparations are almost never 100% pure, which could potentially lead to problems in assay quality and reproducibility. Systems such... 

Arora S, Beaudry C, Bisanz KM et al (2010) A high-content RNAi-screening assay to identify modulators of cholesterol accumulation in Niemann-Pick type C cells. Assay Drug Dev Technol 8 295-320... 

Phototypesetting represented an easier way to compose type. Eady phototypesetters used an optical process, whereby a disk of characters, ia different sizes and typefaces, was spun under computer control. Each character was projected ia turn onto photosensitive film or paper. This was followed by systems where characters drawn on a cathode ray tube (CRT) exposed the photosensitive material. In each case, the operator iateracted with the system at a video screen that only showed the characters of the text (the iaformation content) and codes that iadicated how the characters were to look on paper. An experienced operator was required to obtain high quaUty results. 

ASTM recognizes two types of zinc dust in specification ASTM D 520-51 (reapproved 1976) (143), which includes permissible impurity concentrations. The metallic content of most commercial grades is 95—97%. The zinc oxide content is between 3 and 5% finer dusts contain higher concentrations because of high surface areas. Zinc dusts are manufactured in various size ranges, and a typical commercial dust has an average particle diameter between 4 and 8 p.m. Usually, dusts are screened to be essentially free of particles coarser than 75 p.m (200 mesh). 

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