High-Content Screening HCS

High-throughput automated imaging systems, also sometimes referred to as high content screening (HCS) systems, enable the study of specific cell types within a mixed population of cells (Giuliano, 1997). Some primary cell types are dependent on interactions with other cell types, making their cultivation in isolation impossible, at least at the present time. Even when primary cells are not dependent on interactions with other cell types, primary cell preparations are almost never 100% pure, which could potentially lead to problems in assay quality and reproducibility. Systems such... 

Taylor DL (2010) A personal perspective on high-content screening (HCS) from the beginning. J Biomol Screen 15(7) 720-725... 

Currently, two trends can be observed in the high throughput community miniaturization into the nanoliter dispensing regime and new high content screening (HCS) techniques. Small volume screening (either on 1536-well plates or the re-... 

High-content screening (HCS) is a cell assay method in which phenotypic changes in live cells... 

High-content screening (HCS) has been nsed for early cytotoxicity measurement since 2006 and provides great optimism (Persson et al., 2014). This method has been optimized for stem cell-derived models for the prediction of other organ toxicity. 

TABLE 8.1 Comparison of Outcomes between High Content Screening (HCS) Using HepG2 (Johnson Johnson), Human Hepatocytes (Pfizer), and Zebrafish Phenotypic Hepatotoxicity Screening (Evotec) against FDA Liver Toxicity Knowledge Base (LTKB) DILI Classification... 

High-content screen (HCS) is a phenotypic screening that monitors multiple cellular parameters simultaneously. HCS employs fluorescence-based reagents (antibodies, dyes that bind or localize to a given cell component, sensors) to generate a multicolor fluorescence readout that is usually recorded using automated optical image acquisition devices. 

This screening protocol uses qualitative measures as a read out. However, many high content screening (HCS) tools have been developed which are capable of imaging zebrafish in 96-well formats and could result in more quantitative measures for the phenotype screened. 

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