Heterologous biosynthesis

Trantas E, Panopoulos N, Ververidis F (2009) Metabolic engineering of the complete pathway leading to heterologous biosynthesis of various flavonoids and stilbenoids in Saccharomyces-cerevisiae. MetabEng 11 355-366. doi 10.1016/j.ymben.2009.07.004... 

Keywords Antibiotics screening Enviromnental DNA Heterologous biosynthesis Synthetic biology Systematic engineering... 

Fig. 5.2 Construction and optimization of a synthetic microbe for the natural product heterologous biosynthesis...

Pathway Assembly and Host Redesign for Natural Products Heterologous Biosynthesis... 

Utilization of the MVA pathway for IPP over the DXP pathway (Anthony et al. 2009 Morrone et al. 2010 Tsuruta et al. 2009). However, we found that the DXP pathway owns more potential for IPP supply than MVA pathway through an in silico comparison of the maximum theoretical IPP yields and thermodynamic properties between the DXP and MVA pathways with different hosts and carbon sources. Following woik on heterologous biosynthesis of TaxoFs key intermediate taxadiene via DXP pathway has confirmed our viewpoint (Meng et al. 201 lb). 

Meng HL, Wang Y, Hua Q, Zhang SL, Wang XN (2011b) In silico analysis tmd experimental improvement of taxadiene heterologous biosynthesis in Escherichia coli. Biotechnol Bioprocess Eng 16 205-215... 

Goyer, A. et al.. Folate biosynthesis in higher plants. cDNA cloning heterologous expression and characterization of dihydroneopterin aldolases, Plant. Physiol, 135, 103, 2004. 

Linden, H., Vioque, A., and Sandmann, G., Isolation of a carotenoid biosynthesis gene coding for z-carotene dasaturase from Anabaena PCC 7120 by heterologous complementation, FFMS Microbiol. Lett. 106, 99, 1993. 

In this section, the latest developments and recent publications on the biosynthesis of A9-THC and related cannabinoids as precursors are discussed. Special points of interests are the genetic aspects, enzyme regulation, and the environmental factors that have an influence on the cannabinoid content in the plant. Because of new and innovative developments in biotechnology we will give a short overview of new strategies for cannabinoid production in plant cell cultures and in heterologous organisms. 

Galm, U. and Shen, B. (2006) Expression of biosynthetic gene clusters in heterologous hosts for natural product production and combinatorial biosynthesis. Expert Opinion on Drug Discovery, 1, 409. 

Walker, K., Fujisaki, S., Long, R. and Croteau, R. (2002) Molecular cloning and heterologous expression of the C-13 phenylpropanoid side chain-CoA acyltransferase that functions in Taxol biosynthesis. Proceedings of the National Academy of Sciences of the United States of America, 99, 12715-12720. 

Table 3. Heterologous , expression of PHA biosynthesis genes and related genes ...

There are an overwhelming number of studies which successfully demonstrated heterologous expression of PHA synthesis genes in bacteria it will therefore not be possible to mention them all. Establishment of functional active PHA biosynthesis pathways in E. coli requires not only a PHA synthase but also enzymes that allow the conversion of metabolites, which derive from the provided carbon source, into the R isomers of hydroxyacyl-coenzyme A thioesters that are used as a substrate by the respective PHA synthase. Otherwise, no or only marginal amounts of PHAs are accumulated. 

Table 1. Recombinant bacteria harboring the heterologous PHA biosynthesis genes and synthesized PHA... 

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