Heterologous complementation

Cunningham, EX. Jr. and Gantt, E., Identification of multi-gene families encoding isopentenyl diphosphate isomerase in plants by heterologous complementation in Escherichia coli. Plant Cell Physiol. 41, 119, 2000. 

Linden, H., Vioque, A., and Sandmann, G., Isolation of a carotenoid biosynthesis gene coding for z-carotene dasaturase from Anabaena PCC 7120 by heterologous complementation, FFMS Microbiol. Lett. 106, 99, 1993. 

The complementation experiments in which the A domain of a class 111 E-II is used as the phosphoryl group donor to the B domain of a second E-II molecule with either the same or different sugar specificity, while both are fixed in a membrane matrix, raises some intriguing issues about the association state of these proteins and the kinetics of their interactions. Do E-IIs form stable homologous complexes in the membranes If so, is it necessary to postulate the formation of stable heterologous complexes to explain, for example, the phosphorylation of the B domain of E. coli 11° by the A domain of ll , or can the data be explained by assuming a... 

Stable heterologous complexes are not necessary to explain the limited P-enolpyr-uvate-dependent mannitol phosphorylation kinetic data now available from domain complementation assays transient complexes between domains are sufficient. The challenge remains, however, to visualize how a subcloned A or B domain would be able to transiently associate with an A or B domain on an dimer or tetramer,... 

Xiang L, BS Moore (2002) Inactivation, complementation, and heterologous expression of encP, a novel bacterial phenylalanine ammonia-lyase gene. J Biol Chem 277 32505-32509. 

Yeast does not synthesise cycloartenol or triterpenes, and so approaches to clone plant OSCs by complementation in S. cerevisiae are not feasible because of the lack of appropriate mutants. However, LS-deficient yeast mutants accumulate high levels of 2,3-oxidosqualene, favouring the synthesis of novel cycfisation products generated by heterologous expression of OSCs. The absence of lano-sterol also facilitates analysis of the reaction products. Corey and co-workers isolated a cDNA encoding Arabidopsis thaliana CS by transforming a plant cDNA expression library into such a yeast mutant and screening protein preparations derived from pools of transformants for the ability to synthesise cycloartenol by TLC [39]. 

Delauney, A.J. Verma, D.P.S. (1990a). Isolation of plant genes by heterologous complementation in Escherichia coli In Plant Molecular Biology Manual Supplement, ed. S.B. Gelvin, R.A. Schilperoort D.P.S. Verma, pp. 1-23. Dordrecht Kluwer Academic Publishers. 

R Mazhari-Tabrizi, V Eckert, M Blank, R Muller, D Mumberg, M Funk, RT Schwarz. Cloning and functional expression of glycosyltransferases from parasitic protozoans by heterologous complementation in yeast the dolichol phosphate mannose synthase from Trypanosoma brucei. Biochem J 316 853-858, 1996. 

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