HepG2 hepatocellular carcinoma

The amino acid sequence of the human erythrocyte glucose transporter was deduced from the nucleotide sequence of a cDNA clone in 1985 [106]. Polyclonal antibodies raised against the protein were used to screen a Xgtl I cDNA library prepared from the human hepatocellular carcinoma cell line HepG2. (Like many other transformed... 

Hou et also demonstrated that dihydroartemisinin (39), artemether (40) and artesunate (41) (Fig. 4) were able to inhibit the growth of four human hepatocellular carcinoma cells (HepG2, p53 wild-type Hep3B, p53 null Huh-7, p53 mutant and BEL-7404, p53 mutant). The IC50 values averaged 10.8 /tmol/L (1), 10.6 /tmol/L (39), 21.0 /tmol/L (41), and... 

HepG2 p53 wild-type human hepatocellular carcinoma... 

Cell lines established from human liver cancer cells can be derived from primary hepatocellular carcinoma or hepatoblastoma cells [10]. For example, the well known HepG2 fine was derived from hepatoblastoma cells. Such cells can be employed effectively if the function of normal liver cells has been highly preserved. In practice, however, such cells contain a high proportion of abnormal genetic component, which inhibits their ability to express normal protein synthesis and enzyme activity. Potential problems, such as the loss of liver specific functions and the possibifity of metastasis, which could arise from the use of hepatoma cells have not yet been satisfactorily discussed [13]. 

Feng, B. Effects of extracts from Seeds of Cerbera Manghas on Cell proliferation, cell Cycle progression and apoptosis of human hepatocellular carcinoma HepG2 Cells... 

HEPES Ar-(2-hydroxyethyl)piperazine-A,-(2-ethanesulfonic acid) HepG2 human hepatocellular carcinoma cells HFE a gene for hemochromatosis... 

Buchner RR, Hugh TE, Ember JA, Morgan EL (1995) Expression of functional receptors for human C5a anaphylatoxin (CD88) on the human hepatocellular carcinoma cell line HepG2. Stimulation of acute-phase protein-specific mRNA and protein synthesis by human C5a anaphylatoxin. J Immunol 155 308-315. 

Bae, M. H., Lee, M. J., et al. (1998). Insulin-like growth factor II (IGF-II) secreted from HepG2 human hepatocellular carcinoma cells shows angiogenic activity. Cancer Lett 128(1) 41-6. 

Saint-Pol, A., Bauvy, C., Codogno, P., and Moore, S. E. H. (1997). Transfer of free polyman nos e-type oligosaccharides from the cytosol to lysosomes in cultured human hepatocellular carcinoma HEPG2 cells. J. Cell Biol. 136, 45-59. 

Transcriptional control of iron metabolism has been described, and therefore IRP is imlikely to be the only iron biosensor in mammals. The serum protein ceruloplasmin is transcriptionally regulated by iron in the human hepatocellular carcinoma line HepG2 [48]. Ceruloplasmin is a ferrous iron oxidase (ferroxidase) involved in both transferrin-dependent and -independent iron transport, but appears to facilitate only the latter in the carcinoma cell line. The factor(s) that mediate(s) transcriptional control of ceruloplasmin by iron is not yet known. 

Fig. 2. Plot of PC1-PC2 scores for artemisinin and derivates (training set) with activity against human hepatocellular carcinoma HepG2. More active compounds displayed on the left side (plus sign) while less active ones on the right side (minus sign)...

Now considering our data matrix, PCA was employed looking for a small group of descriptors so that they alone were responsible for classifying all 25 samples into two distinct classes more active and less active. Besides it is desirable to choose uncorrelated descriptors that could be easier to interpret and analyze, trying to associate them to cytotoxicities against human hepatocellular carcinoma HepG2. 

Fig. 4. HCA dendogram for artemisinin and derivatives (training set) with biological activity against human hepatocellular carcinoma HepG2. Plus sign for more active compounds while minus sign for less active ones...

The compound saikosaponin d induced apoptosis in human hepatocellular carcinoma cells (HepG2) through the activation of caspases 3 and 7, resulting in poly(ADP-ribose)-poly-merase (PARP) cleavage. DNA fragmentation was clearly noted at more than 6 hours after exposure to saikosaponin d (Chiang et al. 2003). 

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