Hemolysis immunoglobulin

Selectivity is the ability of an assay to measure the analyte of interest in the presence of other constituents in the sample. Because IAs are often performed without sample extraction, they are more prone to matrix interference than are chromatographic methods with extraction. Matrix interference could come from crossreactivity with structurally similar components in the sample, or from nonspecific binding to structurally dissimilar components in the matrix. The results are high background noise, loss of sensitivity, and inaccurate and nonreproducible data. Sometimes, the problem may only occur in a few exceptional patient samples that have structurally similar components such as unknown metabolites, or dissimilar components from samples with hyperlipidemia, hemolysis, complement components, rheumatoid factors, binding proteins, autoantibodies, and heterophilic anti-immunoglobulin Ab. 

Mild to moderate hemolysis in antiserum resulting from sub-optimal bleeding techniques probably does not interfere with most immunohistochemical staining procedures, but excessive hemolysis should be avoided. If excessive hemolysis or lipemia is encountered, isolation of the immunoglobulin fraction from the antiserum may be necessary. Such isolates will usually appear colorless and clear. Discard all immunochemicals, including antisera and normal non-immune sera contaminated with bacterial growth. Their use in... 

Severe acute hemolysis due to acquisition of red cell alloantibodies from donor serum has been reported (67-69). In other cases, the suggested mechanism of hemolytic anemia after high dosages of intravenous immunoglobulin was the presence of anti-A and/or anti-B antibodies in the plasma product (70). 

Intravascular hemolysis is a rare adverse effect of immunoglobulins, due to the presence of anti-blood group antibodies (119-123). 

The authors considered it probable that the patient s AB blood type had increased the risk of hemolysis and they concluded that patients with severe hemolysis can switch from one batch of intravenous immunoglobulin to another batch of the same product after recovery. 

Padmore RF. Hemolysis upon intravenous immunoglobulin transfusion. Transfus Apher Sci February 2012 46(l) 93-6. 

Rink BD, Gonik B, Chmait RH, O Shaughnessy R. Maternal hemolysis after intravenous immunoglobulin treatment in fetal and neonatal alloimmrme thrombocytopenia. Obstet Gynecol February 2013 121(2 Pt 2 Suppl l) 471-3. 

Shakouri AA, Bahna SL. Acute hemolysis secondary to high-dose intravenous immunoglobulin in a patient with Stevens-Johnson syndrome. Aim llergy Asthma Immunol official Publ Am CoU Allergy, Asthma, Immunol June 2012 108(6) 463-. ... 

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