Anti-blood group

Substances Having Anti-blood Group Specificity. A Discussion on the Specificity of Helix Agglutinins, Vox Sanguinis (1968) 14, 321. 

Intravascular hemolysis is a rare adverse effect of immunoglobulins, due to the presence of anti-blood group antibodies (119-123). 

Since the differences in sequence in the CDR between LAY and POM involve predominantly the first CDR of the light chain, it is probable that some amino acids in this segment are associated with the specificity differences. On this basis the first CDR would be associated with antiprotein (anti-IgG) specificity, and one would thus consider it extremely unlikely that it could also contribute importantly to anticarbohydrate (anti-blood group I) specificity. DRE, GLO, and SIE have identical first CDR, and yet DRE is anti-I while the two others are anti-IgG the second anti-I differs only at position 30 as does one Bence Jones protein, IKE, while two others, TI and WIL, differ at two positions, 30 and 32. POM has the same residue at position 30 as the three Bence Jones proteins and differs from all others only in having He at position 28. Thus position 28 may be predicted to be involved in its specificity for IgGl Asn at position 30 may also be of importance since POM differs from the two anti-I proteins and the two anti IgGs of the Wa idiotype at this position. 

A human serum containing a mono-clonal anti-(blood-group I) antibody has been used to investigate the distribution of blood-group I antigens on erythrocyte membrane components. Different radioactive profiles were revealed by the two radiolabels used with solubilized stroma ( H and In the immuno-precipitates, the predominant I-labelled band had molecular weight 0.9-1.0x10 , whereas the label was associated with a diffuse band (molecular... 

Purification of the anti-(blood-group N) lectin from Vicia graminea seeds Separation of pepsin and chymosin Demonstration of the heterogeneity of rat a-foetoprotein Comparison with kinetic and equililnium dialysis methods for analysis of the binding of nucleotides to staphylococcal nuclease... 

Affinity chromatography of I-labelled solubilized stroma from human erythrocytes and of I-labelled band 3 (the major intrinsic membrane protein) on an anti-(blood group I) adsorbent column has indicated the association of blood-group I activity with band 3." ... 

Blood-group A substance has been partially deacetylated with toluene-p-sulphonic acid. The deacetylated product reacted with a cyclic imidocarbonate derivative of agarose cross-linked with 2,3-dibromopropanol to give a material used as an immunoadsorbent for anti-(blood-group A) antibodies. 

Isolation of prolactin by immunoadsorption Isolation of a pseudo-Tamm-Horsfall glycoprotein by affinity chromatography Isolation of a pseudo-Tamm-Horsfall glycoprotein by affinity chromatography Immunoadsorption of anti-(blood-group A substance) antibody... 

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