Hematoxylin-eosin

Hematoxylin/eosin stains of biopsies of back skin taken 24 h post-chemical peeling, a Glycolic acid peel 70%. Note stratum corneum necrosis... 

Fig. i3.2b-d. Hematoxylin/eosin stains of biopsies of c Twenty-five percent TCA induced mid-epidermal back skin taken 24 b post-chemical peeling, b Salicylic wounding/separation. d Thirty percent TCA peel caused acid 30%. Note mild lymphohistiocytic infiltrate, deep epidermal separation... 

Figure 1.2 shows differently prepared skin membranes. The individual membranes were prepared from freshly excised human abdominal skin, originating from females undergoing reductive surgery. Samples were fixed with xylol, stained with hematoxylin/eosin, and embedded into paraffin. Cross sections with a thickness of 4 fzm were prepared using a microtome. 

Fig. 3. (A andB) Breast carcinoma, in a core biopsy of a patient, pretreatment. Hematoxylin-eosin (H E)-stained histological sections show a poorly differentiated ductal carcinoma with evident nuclear pleomorphism and numerous mitotic figures. (A) H E, magnification x200 (B) H E, magnification x400.

Fig. (8). Increased infiltration of CD3, CD4 and CDS positive cells into the s.c. rechallenge tumor following CVS treatment [44]. BALB/c mice were injected s.c. with 5 x lCr Meth A cells in the right and left flanks on day 0 and 9, respectively. CVS (50 mg/kg) (B,D,F,H) or PBS (A,C,E,G)was injected i.t. into the primary tumor 3 times every 2 days from day 2. Recnallenge tumor sections, including subcutaneous tissues, were obtained 2 days after tumor rechallenge and stained with hematoxylin-eosin (A,B) or immunohistochemically (C-H). a Subcutaneous muscle layer, b interstitial space, c tumor tissue.

Interobserver variation in the grading of VIN has been observed when using hematoxylin-eosin only, MIB-1 monoclonal antibody alone, or combined hematoxylin-eosin and MIB-1 antibody (van Beurden et al., 1999). This antibody is the most versatile proliferation worker for the sections of formalin-fixed and paraffin-embedded tissues. Normal vulvar skin and VIN lesions are fixed with formalin and embedded in paraffin according to standard procedures. 

Incubation with a combination of MIB-1 antibody and hematoxylin-eosin... 

Figure 21.2 Histochemical analysis of / -galactosidase gene expression in liver. Mice were injected with 1.6ml saline containing various amounts of pCMV-LacZ plasmid DNA. Animals were sacrificed eight hours post injection and liver sections were made using cryostat. Sections (A, B, C and D) were stained with X-gal solution followed by eosin for counter-stain. Sections (E, F, G and H) were stained by a standard hematoxylin/eosin staining method. Sections were made from animals each receiving 0 (A, E), 0.5 (B, F), 2.5 (C, G) and 25 pg (D, H) of pCMV-LacZ. (25x). (see Color Plate 14)...

Stain sections with hematoxylin/eosin (see Note 22). 

The method of Koyama et al.12 was used to determine the parakeratotic index of SC. A glass plate was attached to the skin with Scotch tape (Sumitomo 3M, Tokyo, Japan) measuring 25 x 19 mm to remove corneocytes. The adherent horny material was stained with hematoxylin-eosin solution for microscopic inspection of nuclei. The results were scored depending on the number of the nucleated cells in the visual field (0 = none, 1 = small, 2 = relatively large, 3 = very large). 

FIGURE 9.4 Parakeratotic cells detected in the SC obtained by tape stripping. Cells were stained with a hematoxylin-eosin solution. 

Fig.4.3a-f. The phenomenon of infarct maturation is shown as macroscopic appearance on hematoxylin-eosin stained coronal brain sections. Right permanent middle cerebral artery occlusion was performed in rats and histology obtained between 3 h and 7 days. Note the sparse changes at 3 h and lesion shrinkage at 7 days. [Reproduced with permission from Garcia et al. (1993)]... 

After the determination of organ weights and macroscopic examinations the tissues are processed for histo-pathological evaluations. For this they are fixed in formalin or equivalent solutions, or they are frozen (important for the diagnosis of increased fat content in a tissue, e.g. in the liver, organic solvents would dissolved the fat) trimmed to small parts, put into paraffin blocks, cut with a microtome, and stained (hematoxylin-eosin, or special staining for fat and/or collagen etc.). 

Representative sections (6 pm) of each organ were stained with Masson s trichrome and hematoxylin/eosin. 

Histopathology (hematoxylin-eosin stain, periodic acid-Schiff stain, Grocott s methenamine-silver stain) may be helpful in the diagnosis of dermato-phytosis and plays an important role in the diagnosis of subcutaneous mycoses. 

Figure 9.3 Inhibition of intimal hyperplasia by resveratrol in rabbits subjected to endothelial injury by denudation. Groups of eight New Zealand white rabbits, weighting 2.2 to 3.6 kg, were assigned randomly to control (untreated) (M), low (2 mg/kg/d) (L), and high dose (4 mg/kg/d) (H) resveratrol treatment, which was administered intragastrically for 5 weeks beginning 1 week before surgery. A 2-cm segment of injured iliac artery was excised, fixed in 4% paraformalin, embedded in paraffin, and sectioned at5-mm intervals from the proximal to the distal end. Representative sections were stained with hematoxylin/eosin. The external and internal elastic lamina were manually identified. Intimal proliferation index (IPI) was defined as the ratio of intimal area to [intimal+medial] area relative luminal area (RLA) was defined as the ratio of luminal area to [luminal+intimal+medial] area.

Histological examination. Corneas are removed at the end of the experiment as well as at defined intervals after surgery and/or treatment and fixed in formalin for histological examination. Newly formed vessels and the presence of inflammatory cells are detected by hematoxylin/eosin staining or specific immunohistochemical... 

Further, we undertake the rinsing of both substrates in an aqueous flow and immobilize the hematoxylin-poly-L-lysine over them. The PEF spectra of hematoxylin-eosine-poly-L-lysine immobilized between two PSFs (Fig. 2b, spectrum 1) has the maximum near 543 nm as in the previous cases, but HW is much more larger. 

The plasmonic silver films put in pairs as an object-plate and a cover glass allow significantly (up to 3-4 times) increase the staining dyes fluorescence. This effect is observed in the case of optical tuning of LPs. The deposition of hematoxylin-eosine-poly-L-lysine moiety between two plasmonic silver films is carried out as a real biopsy material tincturing in histology. These results may be adopted for clinical assays with the use of biomedical fluorescent microseope. 

A. A low magnification view displays disproportionate tubulointerstitial scarring. There is mild to moderate interstitial chronic inflammation. Prominent tubular calcifications are seen in the upper left and lower right portions of the field. (Hematoxylin eosin,orig.magn. iOOx). 

---