Heidenhain pouches

Conscious cat Gastric fistula and Heidenhain pouch 1 (food and Pgas) 0 (basal and histamine) Bado et al., 1991... 

Conscious Dogs (Chronic Heidenhain-Pouch Fistula... 

Gastric Acid Secretion in Conscious Dogs (Chronic Heidenhain-Pouch Fistula in Dogs)... 

Gastric motility can be measured by balloon manometry of the Heidenhain pouch in the conscious dog. The animals are deprived of food for 18 hours before the experiment, but water is allowed ad libitum. A latex balloon, connected via a polyethylene catheter to a pressure transducer (Statham P 23 BB), is introduced through the fistula cannula into the accessory stomach. Changes in intragastric pressure... 

Jacobson et al. (1966, 1967) studied gastric secretion in relation to mucosal blood flow by an antipyrine clearance technique in conscious dogs with vagally denervated gastric fundic (Heidenhain) pouches. A vagally denervated fundic pouch is so constructed that the entire arterial blood supply is delivered by the splenic artery. A non-cannulating transducer (electromagnetic flowmeter) and a hydraulic occluder were implanted on the vessel. 

The Heidenhain pouch preparation was used by Carter and Grossman (1978), Kauffman et al. (1980) to study the effect of luminal pH on acid secretion evoked by topical and parenteral stimulants and the effect of topical and intravenous 16,16-dimethyl prostaglandin E2 on gastric bicarbonate secretion. 

The Heidenhain pouch technique in dogs has been used for preclinical evaluation of various drugs, such as ... 

Descroix-Vagne et al. (1993) used Heidenhain pouch preparations in cats and rabbits to study the effect of perfusion at pH 5.5 on acid and pepsin secretion... 

Uchida M, Ohba S, Ikarashi Y et al. (1993) Effect of the novel histamine H2 antagonist 5,6-dimethyl-2-[4-[3-(l-piperidinomethyl)phenoxy]-(z)-2-butenylamino]-4(lH)-pyrimidone dihydrochloride on histamine-induced gastric secretion in Heidenhain pouch dogs. Arzneim Forsch/Drug Res 43 873-876... 

Wardle KA, Bingham S, Ellis ES et al. (1996) Selective and functional 5-hydroxytryptamine4 receptor antagonism by SB 207266. Br J Pharmacol 118 665-670 Yamamoto O, Matsunaga Y, Shiba Y et al. (1994) Inhibition of motilin-induced phase III contractions by pentagas-trin in Heidenhain pouch dogs. J Pharmacol Exp Ther 271 1471-1476... 

Yuki H, Nishida A, Miyake A et al. (1997) YM022, a potent and selec-tive gastrin/CCK-B receptor antagonist, inhibits peptone meal-induced gastric secretion in Heidenhain pouch dogs. Dig Dis Sci 42 707-714... 

More recently, Hollander et al. (H27-H30, H32) measured buffer capacity of viscous mucus collected from dog Heidenhain pouches following application of topical mucigogues, and found it to be significant and in the range 9-84 (mean 40) millinormal, when measiu-ed by electrometric titration against HCl. This would indicate that on the average 100 ml mucus may buffer about 40 ml 0.1 normal HCl. 

Hexosamine to Tyrosine Ratio in Mucoprotein H (= Mucoproteose) and Mucoprotein V (= Glandular Mucoprotein) Fractions of Dissolved Gastric Mucin Collected Under Similar Conditions from Heidenhain Pouches of 4 Docs... 

This fractionation method was subsequently used successfully by other investigators (Bll, D2a, Gl, G64, H39, H40, II, 12, N6, N7, P14, S6-S9, W7). Schrager (SIO) 12 years later severely criticized it and considered that measurement of mucosubstances by determination of their peptide moiety was an improper technique for measuring the gastric juice mucosubstances. He simultaneously estimated the tyrosine and hexosamine contents of the mucoprotein and mucoproteose fractions and found no quantitative correlation between the two. He therefore concluded that tyrosine measurement was not adequate for quantitation of these fractions (see Section 2.2). These results are contradicted by recent data of DeGraef (D2a) obtained on Heidenhain pouches which show very satisfactory correlation between mucoprotein and mucoproteose assay by means of tyrosine and hexosamine quantitation (see Fig. I8a). 

Stimulated by milk and raw meat, and at lowest concentration after subcutaneous administration of histamine. In patients with posthemorrhagic anemia, its concentration in gastric juice decreases sharply with multiple blood transfusion and in polycythemia, the concentration increases. As shown in Pavlov and Heidenhain pouches, the formation of this factor is under the influence of the vagus. Denervation of the spleen is reported to lead to its complete, though transient, disappearance from gastric juice. 

Smith, W. O., Hoke, R., Landy, J., Caputto, R., and Wolf, S., The nature of the inhibitory effect of normal human gastric juice on Heidenhain pouch dogs. Gastroenterology 34, 181-187 (1958). 

From biomedical experience with different kinds of gastric acid inhibitors, the conscious dog seems to be the most relevant animal species for the prediction of the antisecretory potential of a test compoimd in humans. Gastric acid secretion can be studied in the dog with a chronic gastric fistula or with a Heidenhain pouch [40]. 

Secretin, an intestinal hormone obtainable from the duodenum, particularly on acidification, has been used clinically as a diagnostic aid in pancreatic disease to stimulate pancreatic secretion. It has been well-established that secretin also inhibits gastric acid secretion. The most recent investigation showed that secretin Inhibited gastrin-stimulated secretion but not that induced by histamine in dogs d.th a Heidenhain pouch.Insulin reD ase by the pancreas is stimulated by secretin in dogs 9 and in man. ... 

The heparinoids have actions as antihistamines. Selected cases of acute hay fever and asthma were helped by intravenous heparin, particularly for the acute attacks. Sufficient clinical improvement was observed to warrant further studies that heparin may provide protection against endogenous histamine. The combination of basic antibiotic and heparin is valuable , possibly due to reduction of toxicity of the antibiotic. While heparin has no effect on the action of histamine on the cat blood pressure, it suppresses histamine-induced gastric acid secretion in Heidenhain pouch dogs - . Chondroitin sulphate reduces the number of gastric ulcers in the Shay rat but polyethylene sulphonates of molecular weights between 15,000 and... 

DMI (desmethylimipramine) is antisecretory in the dog and rat, and man. DMI suppresses 2-DG induced secretion, but not carbachol induced secretion in Heidenhain pouch dogs. This finding and the lack of effect on secretion produced by preganglionic electrical stimulation suggest that the site of DMI action is within the CNS.SS... 

Table 3-1. Hollander s Values for the Electrolyte Composition of 31 Samples of Acetylcholine-Stimulated Mucus, Mucus from Neck Chief Cells, and Anacid Unstimulated Secretion Collected from Dogs Heidenhain Pouches...

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