Hanks’ Balanced Salt Solution

HBSS Hanks balanced salt solution (GibcoBRL, Eggenstein, Germany). 

It must be mentioned here that some of the results discussed in Sect. 4.3 were obtained with cell suspensions in Hanks -balanced salt solution (HBSS) in the absence of plasma proteins. The present author believes that the random-network concept can be applied to the events which happen to lymphocytes, platelets or erythrocytes when they come into direct contact with hydrated material surfaces in the absence of interventing protein. 

Grow the fibroblasts in Ham F-10 medium with 10% fetal calf serum (FCS). Harvest the cells, seed at a density of 30,000 cells and grow to near confluency on cover slips in 6-well plates in Ham F-10 medium with 10% FCS. Remove the medium and wash the cells three times with 2 ml Hanks balanced salt solution. Fix the cells with 3 ml fixative. Seal the plate with tape and store in the refrigerator at 4 C until staining. 

Earle s and Hanks balanced salt solution (BSS) 10 X concentrated, without bicarbonate and glucose... 

Hanks balanced salt solution (HBSS), buffered with 25 mM HEPES at pH 7.2, and containing 0.5% BSA. 

Dilute 120 mg Lucigenin (Sigma Chemical, Deisenhofen, Germany M-8010) in 20 mL HEPES- buffered Hanks balanced salt solution (HBSS Gibco, Grand Island, NY). Lucigenin solution should kept at 4°C. 

Purified eosinophils and neutrophils are suspended at 5 x 104 cells/mL and 5 x 105 cells/mL, respectively, in HEPES- buffered Hanks balanced salt solution, pH 7.4 containing 1 mg/mL BSA (HBSS/BSA). 

Percoll Gradient Preparation Percoll Stock Mix 180 mL Percoll (Pharmacia, Piscataway, NJ), 18 mL 10X Hanks Balanced Salt Solution (HBSS) without Ca,... 

Ten times and single strength HBSS (Hanks balanced salt solution). 

Hanks balanced salt solution (HBSS), pH 7.4. Prepare a IXsolutionfiom the lOX concentrate (obt ned from Gibco, Paisley, Scotland) and adjust the pH with 1.76 mL of 7.5% NaHCOj/lOOmLWarm to37°C (seeNote 3). 

I]ISA-labeled line-10 tumor cells 5 x 10 cells/ml in Hanks balanced salt solution (HESS)... 

The three human IgG products discussed here were purified from Fraction II -I- III paste by slightly different processing methods but all three shared the same final formulation in 0.2 M glycine, pH 4.25. All three were 10% protein solutions, of which 98% was IgG, and their monomer contents were greater than 90%. At production scale, IgG final bulks (pH 4.25) are typically sterile filtered, aseptically filled into sterile final containers and incubated at 20-27°C for 21days-28days. For the virus studies, however, IgG final bulk material was spiked with virus and adjusted to pH 4.3 or 4.5 before incubating at 5, 20, or 23°C, for up to 28 days. Hanks Balance Salt Solution (HBSS) or IgG, adjusted to pH 7, were also spiked with virus and included as positive controls. Aliquots for virus... 

Fig. 4 Kinetics of virus inactivation during pasteurization of 25% albumin. (A) HIV-1, (B) PRV, (C) BVDV, (D) Reo, (E) PPV, and (F) HAV. Virus was spiked into albumin and an aliquot was removed for immediate titration (t = Preheat). Timing of the pasteurization cycle started when the temperature reached 60° C t — Ohr). Unheated albumin and Hanks Balanced Salt Solution (HBSS) were also spiked and incubated at 5°C, as positive controls. Aliquots for virus titration were removed at various times during pasteurization (closed circles = HBSS, 5°C, closed triangles = 25% albumin, 5°C, closed squares = 25% albumin, 60°C, dashed line, no symbol = virus detection limit).

Sample staining buffer (0.1% (w/v) NaNj and 1.0% (w/v) bovine serum albumin in Hanks balanced salt solution)... 

Table2. Composition of Hanks Balanced Salt Solution (HBSS), HBSS-like transport medium (TM) and modified Krebs Bicarbonate Ringer s Solution (KBR).

The quick blot procedure, used for mRNA (not efficient for rRNA or tRNA), is based on the same principle (Costanzi and Gillespie, 1987). A cell pellet is resuspended in Hanks balanced salt solution (Gibco), containing 50 pg/ml of cycloheximide and 10 mM vanadyl-ribonucleoside complexes, to about 10 -10 cells/ml. The cells are incubated for 10 min at 37 C after adding 1 vol. of proteinase K stock solution. Then, 0.05 vol. of 10% Brij 35 is added and mixed, followed by mixing with 0.05 vol. of sodium deoxycholate. The subsequent steps with fresh hot Nal are as for DNA, except that 5 M Nal containing 0.5% Brij is used for dilution. 

BSS, Hanks balanced salt solution BSA, bovine albumin in distilled water BE,- beef extract in... 

Sample preparation 100 pL Cell suspension -I- 100 pL cefoperazone solution -I- 100 pL Hanks balanced salt solution, sonicate 30 min, add 800 pL MeCN, centrifuge at 13000 g... 

Figure 8 Sedimentation of Nylon-6,6 particles coated with a 25 mg/ml bulk concentration of Human Serum Albumin. The particles in each tube are suspended in binary mixtures of Hanks Balanced Salt Solution (HESS) containing different amounts of dimethylsulfoxide, pH 7.2. (Reproduced with permission from Ref. 40. Copyright 1986, Academic Press Inc.)...

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